Cloning and secretory expression of VP2 gene of infectious bursal disease virus in eukaryotic cells

被引：0

作者：

Ghafari, Sh

Shapouri, Seyfiabad M. R. ^{[1
]}

Moatamedi, H. ^{[2
]}

Roayaei, M. ^{[2
]}

Goudarzi, H. ^{[3
]}

机构：

[1] Shahid Chamran Univ Ahvaz, Fac Vet Med, Dept Pathobiol, Ahvaz, Iran

[2] Shahid Chamran Univ Ahvaz, Fac Sci, Dept Biol, Ahvaz, Iran

[3] Razi Vaccine & Serum Res Inst, Dept Avian Med, Karaj, Iran

来源：

IRANIAN JOURNAL OF VETERINARY RESEARCH | 2010年 / 11卷 / 01期

关键词：

Infectious bursal disease; IBDV; VP2; Eukaryotic expression; COS-7 cell line; PROTECTIVE IMMUNE-RESPONSES; DNA VACCINE; ESCHERICHIA-COLI; CHICKENS; CHALLENGE; PROTEIN; SEGMENT;

D O I：

暂无

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

VP2 gene coding region of a vaccinal strain (D78) of infectious bursal disease virus (IBDV) was cloned in a eukaryotic expression vector, pSec Tag2A. The gene was placed downstream of Ig kappa chain leader sequence, under the control of human cytomegalovirus (hCMV) immediate early enhancer and promoter. The construct pSec Tag2A-VP2 was transfected in COS-7 cell line and the expression and secretion of VP2 was assessed by dot blotting and antigen capture ELISA. The antibody used in the immunological assays was a neutralizing monoclonal antibody (1A6) against VP2. Positive reaction with the antibody indicated the construct was functional with respect to expression and secretion of a native VP2.

引用

页码：72 / 77

页数：6

共 30 条

[1] DELETION MAPPING AND EXPRESSION IN ESCHERICHIA-COLI OF THE LARGE GENOMIC SEGMENT OF A BIRNAVIRUS [J].