Two step resonant Mie scattering correction of infrared micro-spectral data: human lymph node tissue

In this manuscript, we report the application of EMSC to correct infrared micro-spectral data recorded from tissue that describe resonant Mie scattering contributions. Small breast micro-metastases previously undetectable using the raw measured spectra were provided clear contrast from the surrounding tissue after signal correction. The technique also proved transferrable, successfully correcting imaging data sets recorded from multiple patients. It is envisaged more robust methods of supervised analysis can now be constructed to automatically classify and diagnose tissue spectra. [GRAPHICS] Microscopic image of a H&E stained lymph node, and a pseudo-colour infrared image obtained from the same section. The yellow areas co-localise with the breast micro-metastasis apparent in the left panel.