Osteoblastic and anti-osteoclastic activities of strontium-substituted silicocarnotite ceramics: In vitro and in vivo studies

被引：83

作者：

Zeng, Junkai ^{[1
]}

Guo, Jingshu ^{[2
]}

Sun, Zhenyu ^{[1
]}

Deng, Fanyan ^{[2
]}

Ning, Congqin ^{[2
]}

Xie, Youzhuan ^{[1
]}

机构：

[1] Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Dept Orthopaed Surg, Shanghai Key Lab Orthopaed Implants,Sch Med, Shanghai, Peoples R China

[2] Chinese Acad Sci, Shanghai Inst Ceram, State Key Lab High Performance Ceram & Superfine, Shanghai 200050, Peoples R China

来源：

BIOACTIVE MATERIALS | 2020年 / 5卷 / 03期

基金：

中国国家自然科学基金;

关键词：

Strontium; Bioceramics; Osteoblastic; Osteoclastic; Silicocarnotite; BONE; SILICATE; DIFFERENTIATION; OSTEOPOROSIS; CHEMISTRY; FRACTURE; CATENIN;

D O I：

10.1016/j.bioactmat.2020.03.008

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Osteoporosis bone defect is a refractory orthopaedic disease which characterized by impaired bone quality and bone regeneration capacity. Current therapies, including antiosteoporosis drugs and artificial bone grafts, are not always satisfactory. Herein, a strontium-substituted calcium phosphate silicate bioactive ceramic (Sr-CPS) was fabricated. In the present study, the extracts of Sr-CPS were prepared for in vitro study and Sr-CPS scaffolds were used for in vivo study. The cytocompatibility, osteogenic and osteoclastogenic properties of Sr-CPS extracts were characterized in comparison to CPS. Molecular mechanisms were also evaluated by Western blot. Sr-CPS extracts were found to promote osteogenesis by upregulating Wnt/beta-catenin signal pathways and inhibit osteoclastogenesis through downregulating NF-kappa B signal pathway. In vivo, micro-CT, histological and histomorphometric observation were conducted after 8 weeks of implantation to evaluate the bone formation using calvarial defects model in ovariectomized rats. Compared with CPS, Sr-CPS significantly promoted critical sized ovariectomy (OVX) calvarial defects healing. Among all the samples, Sr-10 showed the best performance due to a perfect match of bone formation and scaffold degradation rates. Overall, the present study demonstrated that Sr-CPS ceramic can dually modulate both bone formation and resorption, which might be a promising candidate for the reconstruction of osteoporotic bone defect.

引用

页码：435 / 446

页数：12

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