The occurrence of antibiotic-resistant bacteria on the clothes of nursery teachers in daycare centres

被引:2
作者
Zagar, Dominika [1 ]
Zore, Anamarija [1 ]
Torkar, Karmen Godic [1 ]
机构
[1] Univ Ljubljana, Fac Hlth Sci, Dept Sanit Engn, Zdravstvena Pot 5, SI-1000 Ljubljana, Slovenia
关键词
clothes; childcare Centre; antimicrobial resistance; extended-spectrum beta-lactamases; MRSA; public health; SPECTRUM BETA-LACTAMASES; BACILLUS-CEREUS; STAPHYLOCOCCUS-AUREUS; MOLECULAR CHARACTERIZATION; PSEUDOMONAS-AERUGINOSA; ENTEROCOCCUS-FAECIUM; ESCHERICHIA-COLI; ANTIMICROBIAL RESISTANCE; MULTIPLEX PCR; GENES;
D O I
10.1111/jam.15520
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Aims: Childcare facilities act as microenvironments that facilitate and promote the selection, spread and transmission of antibiotic-resistant micro-organisms in the community. We focused on the study of antimicrobial resistance and genetic predispositions for beta-lactamase production in bacterial isolates from nursery teachers' clothing. Methods and Results: Antimicrobial resistance of bacterial strains belonging to Enterobacteriaceae, Enterococcus, Staphylococcus spp., Pseudomonas spp. and Bacillus spp. isolated from 80 samples of nursery teachers' clothing was determined. The selected ES beta L genes were found in 30 (44.1%) of 68 strains examined. The CTX-M type ES beta L determinants were detected in 15.4%, 71.5% and 42.5% of the Enterobacteriaceae, Pseudomonas and Bacillus isolates, respectively. The OXA-type coding genes were detected only in strains of the genera Pseudomonas (57.1%) and Bacillus (48.6%). Thus, most B. cereus strains were sensitive to the recommended antibiotics used to treat infections caused by these bacteria. Methicillin resistance was phenotypically confirmed in 27 (14.6%) of 185 staphylococcal isolates. Four isolates (2.2%) were identified as MRSA. Vancomycin resistance was not observed in any of the staphylococcal and enterococci strains. Conclusions: This study has shown that potential pathogens have been isolated from the clothing of nursery teachers, posing a risk of transmission to children. These clothes should be maintained and properly laundered to avoid cross-contamination and the spread of multidrug-resistant (MDR) bacteria in childcare centres. Significance and impact of the study: This study provides insight into the route of transmission of MDR micro-organisms through the clothing of nursery teachers, to which greater importance should be given in the future. Proper procedures for the cleaning and use of clothing in daycare centres should be clarified and standardized.
引用
收藏
页码:4517 / 4530
页数:14
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