OCT4B Isoform Promotes Anchorage-Independent Growth of Glioblastoma Cells

被引：7

作者：

Choi, Sang-Hun ^{[1
,2
]}

Kim, Jun-Kyum ^{[1
,2
]}

Jeon, Hee-Young ^{[1
,2
]}

Eun, Kiyoung ^{[1
,2
]}

Kim, Hyunggee ^{[1
,2
]}

机构：

[1] Korea Univ, Coll Life Sci & Biotechnol, Dept Biotechnol, Seoul 02841, South Korea

[2] Korea Univ, Inst Anim Mol Biotechnol, Seoul 02841, South Korea

来源：

MOLECULES AND CELLS | 2019年 / 42卷 / 02期

基金：

新加坡国家研究基金会;

关键词：

anchorage-independent growth; cytoplasmic localization; glioblastoma; mechanical stress response; OCT4B; EXTRACELLULAR-MATRIX; EXPRESSION; PROLIFERATION;

D O I：

10.14348/molcells.2018.0311

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

OCT4, also known as POU5F1 (POU domain class 5 transcription factor 1), is a transcription factor that acts as a master regulator of pluripotency in embryonic stem cells and is one of the reprogramming factors required for generating induced pluripotent stem cells. The human OCT4 encodes three isoforms, OCT4A, OCT4B, and OCT4B1, which are generated by alternative splicing. Currently, the functions and expression patterns of OCT4B remain largely unknown in malignancies, especially in human glioblastomas. Here, we demonstrated the function of OCT4B in human glioblastomas. Among the isoform of OCT4B, OCT4B-190 (OCT4B(19kDa)) was highly expressed in human glioblastoma stem cells and glioblastoma cells and was mainly detected in the cytoplasm rather than the nucleus. Overexpression of OCT4B(19kDa) promoted colony formation of glioblastoma cells when grown in soft agar culture conditions. Clinical data analysis revealed that patients with gliomas that expressed OCT4B at high levels had a poorer prognosis than patients with gliomas that expressed OCT4B at low levels. Thus, OCT4B(19kDa) may play a crucial role in regulating cancer cell survival and adaption in a rigid environment.

引用

页码：135 / 142

页数：8

共 32 条

[1] Oct4 Expression in Immature Teratoma of the Ovary Relevance to Histologic Grade and Degree of Differentiation [J].