Detection of bovine viral diarrhoea virus infected cattle -: Testing tissue samples derived from ear tagging using an Erns capture ELISA

被引：37

作者：

Kuhne, S ^{[1
]}

Schroeder, C

Holmquist, G

Wolf, G

Horner, S

Brem, G

Ballagi, A

机构：

[1] Univ Munich, Inst Med Microbiol Infect & Epidem Dis, Munich, Germany

[2] IDEXX Labs, Westbrook, MA USA

[3] IDEXX Scandinavia, Osterbybruk, Sweden

[4] Anim Hlth Serv Thuringia, Bad Langensalza, Thuringia, Germany

[5] Agrobiogen GmbH, Thalmannsdorf, Germany

来源：

JOURNAL OF VETERINARY MEDICINE SERIES B-INFECTIOUS DISEASES AND VETERINARY PUBLIC HEALTH | 2005年 / 52卷 / 06期

关键词：

D O I：

10.1111/j.1439-0450.2005.00861.x

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

A new diagnostic approach testing tissue samples derived from cattle ear tagging for bovine viral diarrhoea virus (BVDV) antigen in a commercially available antigen capture enzyme-linked immunosorbent assay (ACE) was developed. To validate this method, 99 positive and 469 negative samples were tested. With those samples the assay yielded a sensitivity of 100% and specificity of >= 99.6%. Serum and ear tissue samples from 11 persistently infected (PI) BVDV calves were tested. While serum samples were negative after intake of colostrum, the ear tissue samples could be detected positive for BVDV all the time. Testing multiple samples derived from the same ear from PI cattle yielded positive results and low variation. Using cattle ear tags combining the ear tag application with sampling of a small ear tissue plug and testing those tissue samples with an ACE could be a reliable and economic way of BVDV testing.

引用

页码：272 / 277

页数：6

共 29 条

[1] Cytotoxic T-lymphocyte responses in cattle infected with bovine viral diarrhea virus [J].

Beer, M ;

Wolf, G ;

Pichler, J ;

Wolfmeyer, A ;

Kaaden, OR .

VETERINARY MICROBIOLOGY, 1997, 58 (01) :9-22

[2] CONTROL OF BOVINE VIRAL DIARRHEA VIRUS-INFECTION WITHOUT VACCINES [J].

BITSCH, V ;

RONSHOLT, L .

VETERINARY CLINICS OF NORTH AMERICA-FOOD ANIMAL PRACTICE, 1995, 11 (03) :627-&

[3] Intrauterine infection with bovine virus diarrhoea virus on Alpine communal pastures in Switzerland [J].

Braun, U ;

Schönmann, M ;

Ehrensperger, F ;

Hilbe, M ;

Strasser, M .

JOURNAL OF VETERINARY MEDICINE SERIES A-PHYSIOLOGY PATHOLOGY CLINICAL MEDICINE, 1999, 46 (01) :13-17

[4] Immunohistochemistry used as a screening method for persistent bovine viral diarrhea virus infection [J].

Brodersen, BW .

VETERINARY CLINICS OF NORTH AMERICA-FOOD ANIMAL PRACTICE, 2004, 20 (01) :85-+

[5] Direct production losses and treatment costs from bovine viral diarrhoea virus, bovine leukosis virus, Mycobacterium avium subspecies paratuberculosis, and Neospora caninum [J].

Chi, J ;

VanLeeuwen, JA ;

Weersink, A ;

Keefe, GP .

PREVENTIVE VETERINARY MEDICINE, 2002, 55 (02) :137-153

[6] Comparison of ear notch immunohistochemistry, ear notch antigen-capture ELISA, and buffy coat virus isolation for detection of calves persistently infected with bovine viral diarrhea virus [J].

Cornish, TE ;

van Olphen, AL ;

Cavender, JL ;

Edwards, JM ;

Jaeger, PT ;

Vieyra, LL ;

Woodard, LE ;

Miller, DR ;

O'Toole, D .

JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, 2005, 17 (02) :110-117

[7] The detection of bovine viral diarrhoea virus in bulk milk samples by the use of a single-tube RT-PCR [J].

Drew, TW ;

Yapp, F ;

Paton, DJ .

VETERINARY MICROBIOLOGY, 1999, 64 (2-3) :145-154

[8]

FENTON A, 1991, ARCH VIROL, P169

[9]

Frey HR, 1996, PRAKTISCHER TIERARZT, V14, P14

[10] AN ANTIGEN CAPTURE TEST FOR THE DETECTION OF CATTLE VIREMIC WITH BOVINE VIRAL DIARRHEA VIRUS - A COMPARISON WITH BVD VIRUS ISOLATION FROM BUFFY COAT CELLS IN BOVINE KIDNEY-CELLS [J].

GOTTSCHALK, EE ;

GREISERWILKE, I ;

FREY, HR ;

LIESS, B ;

MOENNIG, V .

JOURNAL OF VETERINARY MEDICINE SERIES B-ZENTRALBLATT FUR VETERINARMEDIZIN REIHE B-INFECTIOUS DISEASES AND VETERINARY PUBLIC HEALTH, 1992, 39 (06) :467-472

← 1 2 3 →