The TAGteam motif facilitates binding of 21 sequence-specific transcription factors in the Drosophila embryo

被引:40
作者
Satija, Rahul [2 ]
Bradley, Robert K. [1 ,3 ]
机构
[1] Fred Hutchinson Canc Res Ctr, Div Publ Hlth Sci, Computat Biol Program, Seattle, WA 98109 USA
[2] Univ Oxford, Dept Stat, Oxford OX1 3TG, England
[3] Fred Hutchinson Canc Res Ctr, Div Basic Sci, Seattle, WA 98109 USA
关键词
STEM-CELLS; DNA-REPLICATION; GAGA FACTOR; REGULATORY CIRCUITRY; GENE-EXPRESSION; IN-VITRO; GENOME; CHROMATIN; COMPLEX; SITES;
D O I
10.1101/gr.130682.111
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Highly overlapping patterns of genome-wide binding of many distinct transcription factors have been observed in worms, insects, and mammals, but the origins and consequences of this overlapping binding remain unclear. While analyzing chromatin immunoprecipitation data sets from 21 sequence-specific transcription factors active in the Drosophila embryo, we found that binding of all factors exhibits a dose-dependent relationship with "TAGteam" sequence motifs bound by the zinc finger protein Vielfaltig, also known as Zelda, a recently discovered activator of the zygotic genome. TAGteam motifs are present and well conserved in highly bound regions, and are associated with transcription factor binding even in the absence of canonical recognition motifs for these factors. Furthermore, levels of binding in promoters and enhancers of zygotically transcribed genes are correlated with RNA polymerase II occupancy and gene expression levels. Our results suggest that Vielfaltig acts as a master regulator of early development by facilitating the genome-wide establishment of overlapping patterns of binding of diverse transcription factors that drive global gene expression.
引用
收藏
页码:656 / 665
页数:10
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