Excretory-secretory antigens of Trypanosoma cruzi are potentially useful for serodiagnosis of chronic Chagas' disease

被引:21
作者
Nakazawa, M
Rosa, DS
Pereira, VRA
Moura, MO
Furtado, VC
Souza, WV
Barros, MDDS
Abath, FGC
Gomes, YM
机构
[1] Fiocruz MS, Ctr Pesquisas Aggeu Magalhaes, Dept Imunol, BR-50670420 Recife, PE, Brazil
[2] Univ Pernambuco, Hosp Univ Oswaldo Cruz, Ambulatorio Doenca Chagas, Recife, PE, Brazil
关键词
D O I
10.1128/CDLI.8.5.1024-1027.2001
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The reactivities of sera from chronic chagasic patients against the trypomastigote excreted-secreted antigens (TESA) of Trypanosoma cruzi strains with different biodemes were analyzed by TESA-blot and TESA-enzyme-linked immunosorbent assay (ELISA). Although both tests presented high sensitivity and specificity, TESA-ELISA is more appropriate for screening a larger number of samples.
引用
收藏
页码:1024 / 1027
页数:4
相关论文
共 28 条
[1]   IDENTIFICATION OF A TRYPANOSOMA-CRUZI ANTIGEN THAT IS SHED DURING THE ACUTE PHASE OF CHAGAS-DISEASE [J].
AFFRANCHINO, JL ;
IBANEZ, CF ;
LUQUETTI, AO ;
RASSI, A ;
REYES, MB ;
MACINA, RA ;
ASLUND, L ;
PETTERSSON, U ;
FRASCH, ACC .
MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1989, 34 (03) :221-228
[2]  
Andrade S.G., 1981, Revista do Instituto de Medicina Tropical de Sao Paulo, V23, P245
[3]   Trypanosoma cruzi:: Clonal structure of parasite strains and the importance of principal clones [J].
Andrade, SG .
MEMORIAS DO INSTITUTO OSWALDO CRUZ, 1999, 94 :185-187
[4]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[5]  
Camargo ME, 1992, CHAGAS DIS AM TRYPAN, P165
[6]   CHRONIC + PROGRESSIVE MYOCARDITIS + MYOSITIS IN C3H MICE INFECTED WITH TRYPANOSOMA CRUZI [J].
FEDERICI, EE ;
NEVA, FA ;
ABELMANN, WH .
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1964, 13 (02) :272-+
[7]  
GOLDENBERG S, 1991, Memorias do Instituto Butantan (Sao Paulo), V53, P71
[8]  
Gomes Yara De Miranda, 1995, Revista do Instituto de Medicina Tropical de Sao Paulo, V37, P87
[9]   PCR and sero-diagnosis of chronic Chagas' disease - Biotechnological advances [J].
Gomes, YM .
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 1997, 66 (02) :107-119
[10]  
JAZIN EE, 1991, INFECT IMMUN, V59, P2189