Transcriptomic analysis of differentially expressed genes in the molting gland (Y-organ) of the blackback land crab, Gecarcinus lateralis, during molt-cycle stage transitions

被引:36
作者
Das, Sunetra [1 ,4 ]
Vraspir, Lindsay [1 ]
Zhou, Wen [2 ]
Durica, David S. [3 ]
Mykles, Donald L. [1 ]
机构
[1] Colorado State Univ, Dept Biol, Ft Collins, CO 80523 USA
[2] Colorado State Univ, Dept Stat, Ft Collins, CO 80523 USA
[3] Univ Oklahoma, Dept Biol, Norman, OK 73019 USA
[4] Colorado State Univ, Dept Clin Sci, Ft Collins, CO 80523 USA
来源
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS | 2018年 / 28卷
基金
美国国家科学基金会;
关键词
Crustacea; Transcriptome; Ecdysteroid; Y-organ; Molting; mTOR; Transforming growth factor - beta; Autotomy; Molt-inhibiting hormone; Neuropeptide; Cyclic nucleotide; CAMP; cGMP; Myostatin; Activin; Notch; Wnt; MAP kinase; INHIBITING HORMONE MIH; RNA-SEQ DATA; NITRIC-OXIDE; NEUROENDOCRINE REGULATION; GROWTH COORDINATION; MECHANISTIC TARGET; COUPLES NUTRITION; GUANYLYL CYCLASES; TISSUE EXPRESSION; SIGNALING GENES;
D O I
10.1016/j.cbd.2018.06.001
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A transcriptome of the Gecarcinus lateralis molting gland (Y-organ or YO) contained 48,590 contiguous sequences (contigs) from intermolt (IM), early premolt (EP), mid premolt (MP), late premolt (LP), and postmolt (PM) stages. The YO is kept in the basal state in IM by molt-inhibiting hormone (MIH)/cyclic nucleotide-dependent signaling. YO activation in EP requires down-regulation of MIH signaling and activation of mechanistic target of rapamycin (mTOR)-dependent protein synthesis. Transition of the YO to the committed state in MP requires activin/transforming growth factor-beta (TGFP) signaling. YO repression occurs at the end of LP. A total of 28,179 contigs (58%) showed molt stage-specific changes in gene expression. The largest number of differentially-expressed genes (DEGs) were at the IM/EP (16,142 contigs), LP/PM (18,161 contigs), and PM/IM (8290 contigs) transitions. By contrast, the numbers of DEGs were 372 and 1502 contigs for the EP/MP and MP/LP transitions, respectively. DEG analysis of 23 signal transduction pathways showed significant changes in MIH, mTOR, activin/TGF beta, Notch, MAP kinase, and Wnt signaling. Down-regulation of MIH signaling genes in pre-molt is consistent with reduced MIH sensitivity in MP and LP. Up-regulation of mTOR signaling genes in IM and premolt stages is consistent with its role in YO activation and sustained ecdysteroidogenesis. Up-regulation of activin/TGF beta signaling genes in EP and MP is consistent with the role of a myostatin/activin-like factor in YO commitment. Notch, MAP kinase, and Wnt DEG analysis may indicate possible crosstalk with the MIH, mTOR, and activin/TGF beta pathways to integrate other inputs to control YO ecdysteroidogenesis.
引用
收藏
页码:37 / 53
页数:17
相关论文
共 90 条
[21]   Transcriptome analysis of the molting gland (Y-organ) from the blackback land crab, Gecarcinus lateralis [J].
Das, Sunetra ;
Pitts, Natalie L. ;
Mudron, Megan R. ;
Durica, David S. ;
Mykles, Donald L. .
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS, 2016, 17 :26-40
[22]   Growing backwards: an inverted role for the shrimp ortholog of vertebrate myostatin and GDF11 [J].
De Santis, Christian ;
Wade, Nicholas M. ;
Jerry, Dean R. ;
Preston, Nigel P. ;
Glencross, Brett D. ;
Sellars, Melony J. .
JOURNAL OF EXPERIMENTAL BIOLOGY, 2011, 214 (16) :2671-2677
[23]   Direct effects of hypoxia and nitric oxide on ecdysone secretion by insect prothoracic glands [J].
DeLalio, Leon J. ;
Dion, Sara M. ;
Bootes, Abigail M. ;
Smith, Wendy A. .
JOURNAL OF INSECT PHYSIOLOGY, 2015, 76 :56-66
[24]   Wnt gene family members and their expression profiling in Litopenaeus vannamei [J].
Du, Jiangli ;
Zhang, Xiaojun ;
Yuan, Jianbo ;
Zhang, Xiaoxi ;
Li, Fuhua ;
Xiang, Jianhai .
FISH & SHELLFISH IMMUNOLOGY, 2018, 77 :233-243
[25]   CD-HIT: accelerated for clustering the next-generation sequencing data [J].
Fu, Limin ;
Niu, Beifang ;
Zhu, Zhengwei ;
Wu, Sitao ;
Li, Weizhong .
BIOINFORMATICS, 2012, 28 (23) :3150-3152
[26]  
GERSCH M, 1977, ZOOL JAHRB ALLG ZOOL, V81, P133
[27]   Neuroendocrine regulation of Drosophila metamorphosis requires TGFβ/Activin signaling [J].
Gibbens, Ying Y. ;
Warren, James T. ;
Gilbert, Lawrence I. ;
O'Connor, Michael B. .
DEVELOPMENT, 2011, 138 (13) :2693-2703
[28]   Full-length transcriptome assembly from RNA-Seq data without a reference genome [J].
Grabherr, Manfred G. ;
Haas, Brian J. ;
Yassour, Moran ;
Levin, Joshua Z. ;
Thompson, Dawn A. ;
Amit, Ido ;
Adiconis, Xian ;
Fan, Lin ;
Raychowdhury, Raktima ;
Zeng, Qiandong ;
Chen, Zehua ;
Mauceli, Evan ;
Hacohen, Nir ;
Gnirke, Andreas ;
Rhind, Nicholas ;
di Palma, Federica ;
Birren, Bruce W. ;
Nusbaum, Chad ;
Lindblad-Toh, Kerstin ;
Friedman, Nir ;
Regev, Aviv .
NATURE BIOTECHNOLOGY, 2011, 29 (07) :644-U130
[29]   Environmental effects on fished lobsters and crabs [J].
Green, Bridget S. ;
Gardner, Caleb ;
Hochmuth, Jennifer D. ;
Linnane, Adrian .
REVIEWS IN FISH BIOLOGY AND FISHERIES, 2014, 24 (02) :613-638
[30]   Injury response checkpoint and developmental timing in insects [J].
Hackney, Jennifer F. ;
Cherbas, Peter .
FLY, 2014, 8 (04) :226-231