In vitro angiogenic potency in human microvascular endothelial cells derived from myocardium, lung and skin

被引:4
|
作者
Sievers, Henrieke [1 ]
Bahramsoltani, Mahtab [2 ]
Kaessmeyer, Sabine [1 ]
Plendl, Johanna [1 ]
机构
[1] Free Univ Berlin, Inst Vet Anat, Dept Vet Med, D-14195 Berlin, Germany
[2] Univ Leipzig, Fac Vet Med, Inst Vet Anat, Leipzig, Germany
关键词
Human microvascular endothelial cells; myocardium; lung; foreskin; angiogenic potency; growth medium; GENETIC-HETEROGENEITY; GROWTH-FACTOR; EXPRESSION; VASCULOGENESIS; QUANTITATION; MECHANISMS; CULTURES; PROFILE; ORIGIN; ASSAYS;
D O I
10.3233/CH-2011-1497
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Human microvascular endothelial cells derived from myocardium (HCMEC), lung (HPMEC) and foreskin (HDMEC) showed different angiogenic potency when cultivated in their original growth media provided by the distributors. In order to standardize microenvironmental conditions in an all-in-one assay of angiogenesis the aim of this study was to find one optimal growth medium for the endothelial cells derived from the different organs. Therefore each endothelial cell type was cultivated under identical conditions in the different original growth media as well as in several media formulations of the original growth media. Results reveal that even if cultivated in the same growth medium under exactly the same cultivation conditions - over a prolonged time period of 60 days - the endothelial cells still showed different angiogenic potency. This is due to a combination of extrinsic factors, i.e. the isolation procedure and in particular the growth medium, as well as to intrinsic differences between cells of diverse origin.
引用
收藏
页码:473 / 486
页数:14
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