Utilization of enzyme-substrate interactions in analytical chemistry

Enzymes are capable of a highly specific interaction with a variety of substances including their respective substrates. This review summarizes how such interactions may be used in analytical (bio-)chemistry, e.g., for the elucidation of the binding mechanism, the determination of the binding strength, the carting of the binding site, or the screening of possible substrate/inhibitor molecules. Possible assay formats such as analytical affinity chromatography, affinity capillary electrophoresis (ACE), conventional affinity gel electrophoresis (AEP), and related techniques are discussed together with examples of recent applications. In addition a brief section on enzyme-substrate reactions as tools in analytical chemistry is included, since these are perhaps even more important to analytical (bio-)chemistry. The development and application of bioanalytical systems and especially biosensors in various fields including medicine, biotechnology, agriculture, defense and foodstuffs are considered. (C) 1999 Elsevier Science B.V. All rights reserved.