Circulating Exosomal miR-1290 for Diagnosis of Epithelial Ovarian Cancer

被引:32
作者
Jeon, Hyeji [1 ,2 ]
Seo, Su Min [3 ]
Kim, Tae Wan [2 ,4 ]
Ryu, Jaesung [2 ,4 ]
Kong, Hyejeong [2 ,4 ]
Jang, Si Hyeong [5 ]
Jang, Yong Soo [1 ]
Kim, Kwang Seock [2 ]
Kim, Jae Hoon [6 ]
Ryu, Seongho [3 ]
Jeon, Seob [1 ,2 ]
机构
[1] Soonchunhyang Univ, Cheonan Hosp, Coll Med, Dept Obstet & Gynecol, Cheonan 31151, South Korea
[2] Soonchunhyang Univ, Cheonan Hosp, Soonchunhyang Innovat Convergence Res Ctr, Cheonan 31151, South Korea
[3] Soonchunhyang Univ, Soonchunhyang Inst Medbio Sci SIMS, Cheonan 31151, South Korea
[4] Soonchunhyang Univ, Dept Med Life Sci, Asan 31538, South Korea
[5] Soonchunhyang Univ, Cheonan Hosp, Coll Med, Dept Pathol, Cheonan 31151, South Korea
[6] Yonsei Univ, Coll Med, Gangnam Severance Hosp, Dept Obstet & Gynecol, Seoul 05029, South Korea
关键词
ovarian cancer; exosomal microRNA; biomarker; early diagnosis; MICRORNA; BIOMARKERS; GUIDELINES; CA125; STAGE; HE-4;
D O I
10.3390/cimb44010021
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The aim of the study was to develop a new diagnostic biomarker for identifying serum exosomal miRNAs specific to epithelial ovarian cancer (EOC) and to find out target gene of the miRNA for exploring the molecular mechanisms in EOC. A total of 84 cases of ovarian masses and sera were enrolled, comprising EOC (n = 71), benign ovarian neoplasms (n = 13). We detected expression of candidate miRNAs in the serum and tissue of both benign ovarian neoplasm group and EOC group using real-time polymerase chain reaction. Immunohistochemistry were constructed using formalin fixed paraffin embedded (FFPE) tissue to detect expression level of suppressor of cytokine signaling 4 (SOCS4). In the EOC group, miRNA-1290 was significantly overexpressed in serum exosomes and tissues as compared to benign ovarian neoplasm group (fold change >= 2, p < 0.05). We observed area under the receiver operating characteristic curve (AUC) for miR-1290, using a cut-off of 0.73, the exosomal miR-1290 from serum had AUC, sensitivity, and specificity values of 0.794, 69.2 and 87.3, respectively. In immunohistochemical study, expression of SOCS4 in EOC was lower than that in benign ovarian neoplasm. Serum exosomal miR-1290 could be considered as a biomarker for differential diagnosis of EOC from benign ovarian neoplasm and SOCS4 might be potential target gene of miR-1290 in EOC.
引用
收藏
页码:288 / 300
页数:13
相关论文
共 50 条
[41]   Exosomal miR-34b inhibits proliferation and the epithelial-mesenchymal transition by targeting Notch2 in ovarian cancer [J].
Lu, Shenglian ;
Liu, Wuxia ;
Shi, Hong ;
Zhou, Han .
ONCOLOGY LETTERS, 2020, 20 (03) :2721-2728
[42]   Advances of exosomal miRNAs in the diagnosis and treatment of ovarian cancer [J].
Jun Xiong ;
Fen Fu ;
Feng Yu ;
Xiaoju He .
Discover Oncology, 14
[43]   Advances of exosomal miRNAs in the diagnosis and treatment of ovarian cancer [J].
Xiong, Jun ;
Fu, Fen ;
Yu, Feng ;
He, Xiaoju .
DISCOVER ONCOLOGY, 2023, 14 (01)
[44]   Plasma exosomal miR-320d, miR-4479, and miR-6763-5p as diagnostic biomarkers in epithelial ovarian cancer [J].
Wang, Shiwen ;
Song, Xingguo ;
Wang, Kangyu ;
Zheng, Baibing ;
Lin, Qinghai ;
Yu, Miao ;
Xie, Li ;
Chen, Liang ;
Song, Xianrang .
FRONTIERS IN ONCOLOGY, 2022, 12
[45]   Circulating serum exosomal miR-92a-3p as a novel biomarker for early diagnosis of gastric cancer [J].
Lu, Xu ;
Lu, Jianxin ;
Wang, Siqi ;
Zhang, Yu ;
Ding, Ye ;
Shen, Xianjuan ;
Jing, Rongrong ;
Ju, Shaoqing ;
Chen, Hongmei ;
Cong, Hui .
FUTURE ONCOLOGY, 2021, 17 (08) :907-919
[46]   Upregulated expression of serum exosomal miR-375 and miR-1307 enhance the diagnostic power of CA125 for ovarian cancer [J].
Ying Ying Su ;
Li Sun ;
Zhi Rui Guo ;
Jin Chang Li ;
Ting Ting Bai ;
Xiao Xiao Cai ;
Wen Han Li ;
Ye Fei Zhu .
Journal of Ovarian Research, 12
[47]   Global miRNA Expression Profiling Identifies miR-1290 as Novel Potential oncomiR in Laryngeal Carcinoma [J].
Janiszewska, Joanna ;
Szaumkessel, Marcin ;
Kostrzewska-Poczekaj, Magdalena ;
Bednarek, Kinga ;
Paczkowska, Julia ;
Jackowska, Joanna ;
Grenman, Reidar ;
Szyfter, Krzysztof ;
Wierzbicka, Malgorzata ;
Giefing, Maciej ;
Jarmuz-Szymczak, Malgorzata .
PLOS ONE, 2015, 10 (12)
[48]   Delay in diagnosis of epithelial ovarian cancer [J].
Wikborn, C ;
Pettersson, F ;
Moberg, PJ .
INTERNATIONAL JOURNAL OF GYNECOLOGY & OBSTETRICS, 1996, 52 (03) :263-267
[49]   Ovarian cancer cell invasiveness is associated with discordant exosomal sequestration of Let-7 miRNA and miR-200 [J].
Kobayashi, Miharu ;
Salomon, Carlos ;
Tapia, Jorge ;
Illanes, Sebastian E. ;
Mitchell, Murray D. ;
Rice, Gregory E. .
JOURNAL OF TRANSLATIONAL MEDICINE, 2014, 12
[50]   Ovarian cancer cell invasiveness is associated with discordant exosomal sequestration of Let-7 miRNA and miR-200 [J].
Miharu Kobayashi ;
Carlos Salomon ;
Jorge Tapia ;
Sebastian E Illanes ;
Murray D Mitchell ;
Gregory E Rice .
Journal of Translational Medicine, 12