Yeast stimulation of bone marrow mitosis in the soft-shelled turtle, Pelodiscus sinensis Crother for cytogenetic investigations

被引：0

作者：

Park, IS ^{[1
]}

Kim, EM

Hur, JW

Kartavtseva, IV

机构：

[1] Korea Maritime Univ, Coll Ocean Sci & Technol, Div Marine Environm & Biosci, Fishery Genet & Breeding Lab, Pusan 606791, South Korea

[2] Korea Maritime Univ, Res Inst Marine Sci & Technol, Pusan 606791, South Korea

[3] Russian Acad Sci, Far E Branch, Inst Biol & Soil Sci, Evolut Zool & Genet Lab, Vladivostok 690041, Russia

来源：

KOREAN JOURNAL OF GENETICS | 2005年 / 27卷 / 02期

关键词：

bone marrow; chromosome; mitosis; soft-shelled turtle; yeast stimulation;

D O I：

暂无

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We report a simple method involving the injection of a active yeast to increase the number of mitotic cells in the bone marrow tissue of the soft-shelled turtle, Pelodiscus sinensis Crother. Intramuscular injections of a suspension of active yeast into the hind leg region elevated the bone marrow mitotic index by as much as 10-fold. The metaphases induced using this method showed excellent chromosome spreading when air-dried. This method should prove useful to investigators who wish to use bone marrow as a source of chromosomes for soft-shelled turtle cytogenetic studies.

引用

页码：141 / 144

页数：4

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