Microbiological Safety Cabinets (MSC) designed to minimize hazards inherent in work with agents assigned to biosafety levels 1, 2, 3, or 4by keeping hazards work in controled area via filtered air flow. This work defines the tests that shall be passed by such cabinetry to meet the EN 12469 standard. In this work, 5 different types of MSCs' were tested according to the EN 12469 standards and 5 different test methods were analysed.