Molecular cloning, sequence characterization and expression pattern of BURP domain-containing protein 17 gene from watermelon (Citrullus lanatus)

The complete mRNA sequence of the watermelon BURP domain-containing protein 17 gene was amplified through rapid amplification of cDNA ends (RACE) method. The full-length mRNA was 1,085 bp containing a 996 bp open reading frame which encodes a protein of 331 amino acids. Sequence analysis revealed that watermelon BURP domain-containing protein 17 protein shares high homology with the BURP domain-containing protein 17 of cucumber (82%), muskmelon (82%), apple (58%), soybean(57%) and wine grape (54%). Phylogenetic analysis revealed that watermelon BURP domain-containing protein 17 gene has a closer genetic relationship with the BURP domain-containing protein 17 gene of cucumber and muskmelon. Tissue expression profile analysis was carried out and results indicated that the watermelon BURP domain-containing protein 17 gene was highly expressed in root and stem, moderately expressed in flower and leaf, and weakly expressed in fruit. These results established the primary foundation to understand the watermelon BURP domain-containing protein 17 gene.