Blood-induced differential gene expression in Anopheles dirus evaluated using RNA sequencing

被引：3

作者：

Mongkol, W. ^{[1
]}

Nguitragool, W. ^{[2
]}

Sattabongkot, J. ^{[3
]}

Kubera, A. ^{[1
,4
]}

机构：

[1] Kasetsart Univ, Dept Genet, Fac Sci, 50 Ngamwongwan Rd, Bangkok 10900, Thailand

[2] Mahidol Univ, Fac Trop Med, Dept Mol Trop Med & Genet, Bangkok, Thailand

[3] Mahidol Univ, Fac Trop Med, Mahidol Vivax Res Unit, Bangkok, Thailand

[4] Kasetsart Univ, Ctr Adv Studies Trop Nat Resources, Bangkok, Thailand

来源：

MEDICAL AND VETERINARY ENTOMOLOGY | 2018年 / 32卷 / 04期

关键词：

Anopheles dirus; blood-induced genes; malaria; RNA sequencing; MALARIA VECTOR; BODY-SIZE; MOSQUITO; GAMBIAE; TRYPSIN; MIDGUT; DEGRADATION; SECRETION; CULICIDAE; DIPTERA;

D O I：

10.1111/mve.12310

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

Malaria parasites are transmitted through blood feeding by female Anopheline mosquitoes. Unveiling the blood-feeding process will improve understanding of vector biology. Anopheles dirus (Diptera: Culicidae) is one of the primary malaria vectors in the Greater Mekong Subregion, the epicentre of malaria drug resistance. In this study, differential gene expression between sugar- and blood-fed An. dirus was investigated by RNA sequencing (RNA-seq). A total of 589 transcripts were found to be upregulated and 703 transcripts downregulated as a result of blood feeding. Transcriptional differences were found in genes involved in blood digestion, peritrophic matrix formation, oogenesis and vitellogenesis. The expression levels of several genes were validated by quantitative reverse transcription polymerase chain reaction. The present results provide better understanding of An. dirus biology in relation to its blood feeding.

引用

页码：399 / 406

页数：8

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