CREB is a regulatory target for the protein kinase Akt/PKB

被引：830

作者：

Du, KY ^{[1
]}

Montminy, M ^{[1
]}

机构：

[1] Harvard Univ, Sch Med, Dept Cell Biol, Joslin Diabet Ctr,Res Div, Boston, MA 02215 USA

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 1998年 / 273卷 / 49期

关键词：

D O I：

10.1074/jbc.273.49.32377

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The nuclear factor CREB stimulates the expression of cellular genes following its protein kinase A-mediated phosphorylation at Ser-133. Ser-133 phosphorylation, in turn, activates target gene expression by promoting recruitment of the co-activator CBP, Recent studies showing that CREE and its paralog CREM are required for survival of certain cell types prompted us to examine whether CREB is a nuclear target for activation via the growth factor-dependent Ser/Thr kinase Akt/PKB. When overexpressed in serum-stimulated cells, Akt/PKB potently induced Ser-133 phosphorylation of CREB and promoted recruitment of CBP, Correspondingly, Akt/PKB stimulated target gene expression via CREB in a phospho(Ser-133) dependent manner. Akt/PKB induced CREB activity only in response to serum stimulation, and this effect was suppressed by the phosphatidylinositol 3-kinase inhibitor LY 294002. Our results support the notion that Akt/PKB promotes cell survival, at least in part, by stimulating the expression of cellular genes via the CREB/CBP nuclear transduction pathway.

引用

页码：32377 / 32379

页数：3

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