Performance evaluation of an in-house human immunodeficiency virus type-1 protease-reverse transcriptase genotyping assay in Cameroon

被引：43

作者：

Fokam, J. ^{[2
,3
]}

Salpini, R. ^{[1
]}

Santoro, M. M. ^{[1
]}

Cento, V. ^{[1
]}

D'Arrigo, R. ^{[4
]}

Gori, C. ^{[4
]}

Perno, C. F. ^{[1
,4
]}

Colizzi, V. ^{[1
,2
]}

Nanfack, A. ^{[2
]}

Gwom, L. C. ^{[2
,3
]}

Cappelli, G. ^{[2
,5
]}

Takou, D. ^{[2
]}

机构：

[1] Univ Roma Tor Vergata, Dept Expt Med & Biochem Sci, I-00133 Rome, Italy

[2] CIRCB Chantal BIYA Int Res Ctr Res HIV AIDS Prev, Yaounde, Cameroon

[3] Univ Yaounde, Fac Med & Biomed Sci, Yaounde, Cameroon

[4] IRCSS L Spallanzani, Rome, Italy

[5] CNR, Rome, Italy

来源：

ARCHIVES OF VIROLOGY | 2011年 / 156卷 / 07期

关键词：

ANTIRETROVIRAL DRUG-RESISTANCE; HIV GENETIC DIVERSITY; SYSTEM; INFECTION; SURVEILLANCE; DIAGNOSIS; THERAPY; IMPACT;

D O I：

10.1007/s00705-011-0982-3

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Most commercial HIV-1 genotyping assays are hampered by high cost in resource-limited settings. Moreover, their performance might be influenced over time by HIV genetic heterogeneity and evolution. An in-house genotyping protocol was developed, and its sequencing performance and reproducibility were compared to that of ViroSeq (TM). One hundred ninety plasma samples from HIV-1-infected subjects in Cameroon, a resource-limited setting with a high HIV genetic variability, were processed for pol gene sequencing with an in-house protocol, ViroSeq (TM), or both. Only non-B subtypes were found. The in-house sequencing performance was 98.7% against 92.1% with ViroSeq (TM). Among 36 sequence pairs obtained using both assays, the overall rate of discordant amino acid positions was negligible (0.24%). With its high sensitivity and reproducibility, as well as its affordable cost (about half of ViroSeq (TM): 92 a,not sign vs. 217 a,not sign), this in-house assay is a suitable alternative for HIV-1 genotyping in resource-limited and/or in high-genetic-diversity settings.

引用

页码：1235 / 1243

页数：9

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