Detection of PCR-amplified fungal DNA by using a PCR-ELISA system

被引：88

作者：

Loffler, J

Hebart, H

Sepe, S

Schumacher, U

Klingebiel, T

Einsele, H

机构：

[1] Univ Tubingen, Med Klin, Abt 2, Lab Dr Einsele, D-72086 Tubingen, Germany

[2] Univ Tubingen, Inst Hyg, Abt Med Mikrobiol, Tubingen, Germany

[3] Univ Tubingen, Kinderklin, D-7400 Tubingen, Germany

来源：

MEDICAL MYCOLOGY | 1998年 / 36卷 / 05期

关键词：

detection; fungal DNA; hybridization; plate assay;

D O I：

10.1080/02681219880000441

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

In order to speed up and standardize polymerase chain reaction (PCR)-based detection of medically important fungi in clinical samples we established a combination of commercially available kits for DNA extraction, PCR amplification and detection of the amplicons. The PCR plate assay proved to be as sensitive and specific as our previously published assay (5 cfu ml(-1) blood). Moreover, in a selected group of patients, all patients with proven and probable invasive fungal infection were found to be PCR-positive. Thus the PCR plate assay was found to be a sensitive, technically simplified and standardized method with potential for adaptation to automation.

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收藏

页码：275 / 279

页数：5

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