Crassostrea gigas-Based Bioactive Peptide Protected Thrombin-Treated Endothelial Cells against Thrombosis and Cell Barrier Dysfunction

被引：3

作者：

Cheng, Shuzhen ^{[1
,2
]}

Wu, Di ^{[1
]}

Yuan, Lushun ^{[3
]}

Liu, Hanxiong ^{[1
]}

El-Seedi, Hesham R. ^{[4
]}

Du, Ming ^{[1
]}

机构：

[1] Dalian Polytech Univ, Sch Food Sci & Technol, Collaborat Innovat Ctr Seafood Deep Proc, Natl Engn Res Ctr Seafood, Dalian 116034, Peoples R China

[2] Leiden Univ Med Ctr, Div Thrombosis & Hemostasis, Einthoven Lab Vasc & Regenerat Med, NL-2333 ZA Leiden, Netherlands

[3] Leiden Univ Med Ctr, Dept Internal Med, Einthoven Lab Vasc & Regenerat Med, Nephrol, NL-2333 ZA Leiden, Netherlands

[4] Uppsala Univ, Dept Med Chem, Pharmacognosy Grp, Biomed Ctr, S-75123 Uppsala, Sweden

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2022年 / 70卷 / 31期

关键词：

thrombin; endothelial cells; oyster-derived bioactive dodecapeptide; protein kinase; inflammation; MESSENGER-RNA LEVELS; PROSTACYCLIN PRODUCTION; ACTIVATION; MARKERS; ACTIN;

D O I：

10.1021/acs.jafc.2c02435

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

The activation of thrombin-treated endothelial cells resulted in disruption of the vascular tissues. A novel oyster-derived bioactive dodecapeptide (IEELEELEAER, P-2-CG) was reported to protect the human umbilical vein endothelial cells and their barrier function via the decrease of VE-cadherin disruption and the restoration of the F-actin arrangement. The promotion of the extrinsic pathway in this case triggers the release of tissue factors that occurs on the surface of the endothelial cells, thus changing the antithrombotic to prothrombotic. P-2-CG induced accordingly a prolongation of plasma clotting time and thrombin generation time, following the alteration of the antithrombotic phenotype. Furthermore, the antithrombotic activity of P-2-CG was also supported by the reduction of FXa and the inhibition of other factors release, for instance, inflammation factors, ROS, etc. In addition to its antithrombogenic role, P-2-CG displayed anti-inflammatory and antioxidant properties via the mitogen-activated protein kinase cascades and central signaling pathways as shown in an in vitro model of endothelial dysfunction.

引用

页码：9664 / 9673

页数：10