3D SILICON ELECTRODES WITH BUILT-IN GLASS CAPILLARIES FOR DIELECTROPHORETIC SINGLE-CELL POSITIONING AND ANAYLISIS

被引：0

作者：

Luo, Y. ^{[1
,2
]}

Yobas, L. ^{[1
]}

机构：

[1] Hong Kong Univ Sci & Technol, Dept Elect & Comp Engn, Hong Kong, Hong Kong, Peoples R China

[2] CALTECH, Dept Med Engn, Pasadena, CA USA

来源：

2015 TRANSDUCERS - 2015 18TH INTERNATIONAL CONFERENCE ON SOLID-STATE SENSORS, ACTUATORS AND MICROSYSTEMS (TRANSDUCERS) | 2015年

关键词：

Single-cell analysis; dielectrophoresis; 3D microelectrodes; microcapillaries; CHIP;

D O I：

暂无

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

We present microfluidic designs for dielectrophoretic positioning of single cells and their subsequent analysis based on 3D silicon electrodes featuring built-in capillaries. The designs are presented through numerical simulations as well as experimental validations showing effectiveness of dielectrophoretic forces in trapping single cells. Single-cell experiments are also demonstrated including viability assays and ionophore-mediated intracellular calcium flux assays. Seal resistance measurements between the capillaries and cells trapped, which are essential for patch-clamp assays, are reported.

引用

页码：540 / 543

页数：4

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