3D SILICON ELECTRODES WITH BUILT-IN GLASS CAPILLARIES FOR DIELECTROPHORETIC SINGLE-CELL POSITIONING AND ANAYLISIS

被引：0
|
作者：
Luo, Y. [1 ,2 ]
Yobas, L. [1 ]
机构：
[1] Hong Kong Univ Sci & Technol, Dept Elect & Comp Engn, Hong Kong, Hong Kong, Peoples R China
[2] CALTECH, Dept Med Engn, Pasadena, CA USA
来源：
2015 TRANSDUCERS - 2015 18TH INTERNATIONAL CONFERENCE ON SOLID-STATE SENSORS, ACTUATORS AND MICROSYSTEMS (TRANSDUCERS) | 2015年
关键词：
Single-cell analysis; dielectrophoresis; 3D microelectrodes; microcapillaries; CHIP;
D O I：
暂无
中图分类号：
TM [电工技术]; TN [电子技术、通信技术];
学科分类号：
0808 ; 0809 ;
摘要：
We present microfluidic designs for dielectrophoretic positioning of single cells and their subsequent analysis based on 3D silicon electrodes featuring built-in capillaries. The designs are presented through numerical simulations as well as experimental validations showing effectiveness of dielectrophoretic forces in trapping single cells. Single-cell experiments are also demonstrated including viability assays and ionophore-mediated intracellular calcium flux assays. Seal resistance measurements between the capillaries and cells trapped, which are essential for patch-clamp assays, are reported.
引用
收藏
页码：540 / 543
页数：4
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