Core Genome Multilocus Sequence Typing and Prediction of Antimicrobial Susceptibility Using Whole-Genome Sequences of Escherichia coli Bloodstream Infection Isolates

被引：3

作者：

Banerjee, Ritu ^{[1
]}

Cunningham, Scott A. ^{[2
]}

Beisken, Stephan ^{[3
]}

Posch, Andreas E. ^{[3
]}

Johnston, Brian ^{[4
]}

Johnson, James R. ^{[4
]}

Patel, Robin ^{[2
]}

机构：

[1] Vanderbilt Univ, 221 Kirkland Hall, Nashville, TN 37235 USA

[2] Mayo Clin, Rochester, MN USA

[3] Ares Genet GmbH, Vienna, Austria

[4] Univ Minnesota, Vet Affairs Med Ctr, Minneapolis, MN USA

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2021年 / 65卷 / 11期

基金：

美国国家卫生研究院;

关键词：

Escherichia coli; antibiotic susceptibility prediction; molecular typing; whole-genome sequencing; STRAIN;

D O I：

10.1128/AAC.01139-21

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

In total, 50 Escherichia coli bloodstream isolates from the clinical laboratory and 12 E. coli isolates referred for pulsed-field gel electrophoresis (PFGE) were sequenced, assessed for clonality using core genome multilocus sequence typing (cgMLST), and evaluated for genomic susceptibility predictions using ARESdb. Results of sequence typing using whole-genome sequencing (WGS)-based MLST and sequence type (ST)-specific PCR were identical. Overall categorical agreement between genotypic (ARESdb) and phenotypic susceptibility testing for 62 isolates and 11 antimicrobial agents was 91%. Among the referred isolates, high major error rates were found for ceftazidime, cefepime, and piperacillin-tazobactam.

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