Comparative Analysis of B-Cell Receptor Repertoires Induced by Live Yellow Fever Vaccine in Young and Middle-Age Donors

被引：19
作者：
Davydov, Alexey N. [1 ]
Obraztsova, Anna S. [2 ,3 ]
Lebedin, Mikhail Y. [4 ]
Turchaninova, Maria A. [4 ,5 ,6 ]
Staroverov, Dmitriy B. [4 ,5 ]
Merzlyak, Ekaterina M. [4 ,5 ]
Sharonov, George, V [4 ,6 ]
Kladova, Olga [5 ]
Shugay, Mikhail [3 ,4 ,5 ,6 ]
Britanova, Olga, V [4 ,5 ,6 ]
Chudakov, Dmitriy M. [1 ,3 ,4 ,5 ,6 ]
机构：
[1] Cent European Inst Technol, Adapt Immun Grp, Brno, Czech Republic
[2] Lomonosov Moscow State Univ, Fac Bioengn & Bioinformat, Moscow, Russia
[3] Ctr Life Sci, Skolkovo Inst Sci & Technol, Moscow, Russia
[4] Shemyakin & Ovchinnikov Inst Bioorgan Chem, Genom Adapt Immun Dept, Moscow, Russia
[5] Pirogov Russian Natl Res Med Univ, Dept Mol Technol, Moscow, Russia
[6] Privotzhsky Res Med Univ, Lab Genom Antitumor Adapt Immun, Nizhnii Novgorod, Russia
来源：
FRONTIERS IN IMMUNOLOGY | 2018年 / 9卷
基金：
俄罗斯科学基金会;
关键词：
immunoglobulin repertoire; vaccination; age; yellow fever; plasma cell; ANTIBODY REPERTOIRE; INFLUENZA VACCINATION; IMMUNE-SYSTEM; ELDERLY INDIVIDUALS; LINEAGE TREES; OLDER-ADULTS; RESPONSES; SELECTION; DYNAMICS; DIVERSITY;
D O I：
10.3389/fimmu.2018.02309
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Age-related changes can significantly alter the state of adaptive immune system and often lead to attenuated response to novel pathogens and vaccination. In present study we employed 5'RACE UMI-based full length and nearly error-free immunoglobulin profiling to compare plasma cell antibody repertoires in young (19-26 years) and middle-age (45-58 years) individuals vaccinated with a live yellow fever vaccine, modeling a newly encountered pathogen. Our analysis has revealed age-related differences in the responding antibody repertoire ranging from distinct IGH CDR3 repertoire properties to differences in somatic hypermutation intensity and efficiency and antibody lineage tree structure. Overall, our findings suggest that younger individuals respond with a more diverse antibody repertoire and employ a more efficient somatic hypermutation process than elder individuals in response to a newly encountered pathogen.
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页数：11
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