LASP1 promotes glioma cell proliferation and migration and is negatively regulated by miR-377-3p

被引：30

作者：

Liu, Ying ^{[1
]}

Gao, Yang ^{[2
]}

Li, Deheng ^{[2
]}

He, Luyun ^{[2
]}

Lao, I. W. ^{[3
]}

Hao, Bin ^{[2
]}

Chen, Xin ^{[2
]}

Cao, Yiqun ^{[2
]}

机构：

[1] Second Mil Med Univ, Changhai Hosptial, Dept Blood Transfus, Shanghai 200433, Peoples R China

[2] Fudan Univ, Shanghai Med Coll, Dept Oncol, Dept Neurosurg,Shanghai Canc Ctr, Shanghai 200032, Peoples R China

[3] Fudan Univ, Shanghai Canc Ctr, Dept Pathol, Shanghai 200032, Peoples R China

来源：

BIOMEDICINE & PHARMACOTHERAPY | 2018年 / 108卷

基金：

上海市自然科学基金;

关键词：

LASP1; miR-377-3p; PI3K/AKT; Glioma; Proliferation; Migration; SH3; PROTEIN-1; COLORECTAL-CANCER; NUCLEAR-LOCALIZATION; LIM; METASTASIS; PATHWAY; GLIOBLASTOMA; EXPRESSION; CARCINOMA; SURVIVAL;

D O I：

10.1016/j.biopha.2018.09.068

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Glioma is one of the most aggressive and lethal human cancers with a low cure rate. LASP1 plays an oncogenic role in multiple human cancers; however, its role in glioma remains largely unknown. Here, we found that LASP1 was highly expressed in glioma tissue samples. Functionally, knockdown of LASP1 significantly suppressed glioma cell proliferation and migration in vitro and tumorigenicity in vivo. These effects were found to be mechanistically associated with suppression of AKT activity. Furthermore, we identified LASP1 as a direct target of miR-377-3p. Overexpression of miR-377-3p reduced the expression of LASP1 and suppressed the proliferation and migration of glioma cells. Restoration of LASP1 expression in miR-377-3p-overexpressing cells attenuated the inhibition of glioma cell malignancy and reversed the dephosphorylation of AKT. Taken together, our results suggest that LASP1 activates the PI3K/AKT signaling pathway and is downregulated by miR-377-3p during glioma progression. These data provide a new possible therapeutic target in glioma.

引用

页码：845 / 851

页数：7

共 25 条

[11] Conditional Probability of Survival in Patients With Newly Diagnosed Glioblastoma [J].

Polley, Mei-Yin C. ;

Lamborn, Kathleen R. ;

Chang, Susan M. ;

Butowski, Nicholas ;

Clarke, Jennifer L. ;

Prados, Michael .

JOURNAL OF CLINICAL ONCOLOGY, 2011, 29 (31) :4175-4180

[12] Lasp-1, a novel type of actin-binding protein accumulating in cell membrane extensions [J].

Schreiber, VR ;

Moog-Lutz, C ;

Regnier, CH ;

Chenard, MP ;

Boeuf, H ;

Vonesch, JL ;

Tomasetto, C ;

Rio, MC .

MOLECULAR MEDICINE, 1998, 4 (10) :675-687

[13] Long non-coding RNA NEAT1 promotes non-small cell lung cancer progression through regulation of miR-377-3p-E2F3 pathway [J].

Sun, Chengcao ;

Li, Shujun ;

Zhang, Feng ;

Xi, Yongyong ;

Wang, Liang ;

Bi, Yongyi ;

Li, Dejia .

ONCOTARGET, 2016, 7 (32) :51784-51814

[14] Suppression of LASP-1 attenuates the carcinogenesis of prostatic cancer cell lines: Key role of the NF-κB pathway [J].

Sun, Wendong ;

Guo, Liqiang ;

Shao, Guangfeng ;

Liu, Xiangguo ;

Guan, Yong ;

Su, Ling ;

Zhao, Shengtian .

ONCOLOGY REPORTS, 2017, 37 (01) :341-347

[15] MED27 promotes melanoma growth by targeting AKT/MAPK and NF-κB/iNOS signaling pathways [J].

Tang, Ranran ;

Xu, Xiangdong ;

Yang, Wenjing ;

Yu, Wendan ;

Hou, Shuai ;

Xuan, Yang ;

Tang, Zhipeng ;

Zhao, Shilei ;

Chen, Yiming ;

Xiao, Xiangsheng ;

Huang, Wenlin ;

Guo, Wei ;

Li, Man ;

Deng, Wuguo .

CANCER LETTERS, 2016, 373 (01) :77-87

[16] LASP-1 (MLN-50) DEFINES A NEW LIM PROTEIN SUBFAMILY CHARACTERIZED BY THE ASSOCIATION OF LIM AND SH3 DOMAINS [J].

TOMASETTO, C ;

MOOGLUTZ, C ;

REGNIER, CH ;

SCHREIBER, V ;

BASSET, P ;

RIO, MC .

FEBS LETTERS, 1995, 373 (03) :245-249

[17] Role of LIM and SH3 Protein 1 (LASP1) in the Metastatic Dissemination of Medulloblastoma [J].

Traenka, Christopher ;

Remke, Marc ;

Korshunov, Andrey ;

Bender, Sebastian ;

Hielscher, Thomas ;

Northcott, Paul A. ;

Witt, Hendrik ;

Ryzhova, Marina ;

Felsberg, Joerg ;

Benner, Axel ;

Riester, Stephanie ;

Scheurlen, Wolfram ;

Grunewald, Thomas G. P. ;

von Deimling, Andreas ;

Kulozik, Andreas E. ;

Reifenberger, Guido ;

Taylor, Michael D. ;

Lichter, Peter ;

Butt, Elke ;

Pfister, Stefan M. .

CANCER RESEARCH, 2010, 70 (20) :8003-8014

[18] LIM and SH3 Protein 1 Induces TGFβ-Mediated Epithelial-Mesenchymal Transition in Human Colorectal Cancer by Regulating S100A4 Expression [J].

Wang, Hui ;

Shi, Jiaolong ;

Luo, Yuhao ;

Liao, Qing ;

Niu, Ya ;

Zhang, Feifei ;

Shao, Ziyun ;

Ding, Yanqing ;

Zhao, Liang .

CLINICAL CANCER RESEARCH, 2014, 20 (22) :5835-5847

[19] miR-133a represses tumour growth and metastasis in colorectal cancer by targeting LIM and SH3 protein 1 and inhibiting the MAPK pathway [J].

Wang, Hui ;

An, Hongying ;

Wang, Bin ;

Liao, Qing ;

Li, Weidong ;

Jin, Xuejun ;

Cui, Shuzhong ;

Zhang, Yajie ;

Ding, Yanqing ;

Zhao, Liang .

EUROPEAN JOURNAL OF CANCER, 2013, 49 (18) :3924-3935

[20] LIM and SH3 protein 1, a promoter of cell proliferation and migration, is a novel independent prognostic indicator in hepatocellular carcinoma [J].

Wang, Hui ;

Li, Weidong ;

Jin, Xuejun ;

Cui, Shuzhong ;

Zhao, Liang .

EUROPEAN JOURNAL OF CANCER, 2013, 49 (04) :974-983

← 1 2 3 →