Dihydropyridine-derived BODIPY probe for detecting exogenous and endogenous nitric oxide in mitochondria

被引：34

作者：

Gao, Chang ^{[1
,2
,3
]}

Lin, Lan ^{[1
]}

Sun, Wan ^{[2
]}

Tan, Zheng-Li ^{[2
]}

Huang, Jun-Ru ^{[3
]}

He, Lan ^{[1
]}

Lu, Zhong-Lin ^{[2
]}

机构：

[1] Inst Chem Drug Control, Natl Inst Food & Drug Control, TianTan XiLi 2, Beijing 100050, Peoples R China

[2] Beijing Normal Univ, Coll Chem, Xinjiekouwai St 19, Beijing 100875, Peoples R China

[3] China Pharmaceut Univ, Sch Pharm, Nanjing 211198, Jiangsu, Peoples R China

来源：

TALANTA | 2018年 / 176卷

关键词：

BODIPY; Nitric oxide; Fluorescent sensor; Dihydropyridine; Mitochondria; TARGETABLE FLUORESCENT-PROBE; LIVING CELLS; RATIONAL DESIGN; AQUEOUS-SOLUTION; TURN-ON; NO; LYSOSOME; SENSORS; NITRATE;

D O I：

10.1016/j.talanta.2017.08.044

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A mitochondria-targetable probe Mito-DHP for nitric oxide (NO) was designed and synthesized by introducing dihydropyridine and triphenylphosphonium (TPP) moieties into boron dipyrromethene (BODIPY) dye. Mito-DHP was able to effectively detect nitric oxide through the aromatization of dihydropyridine to fluorescent pyridine product under oxygen-free conditions. The probe Mito-DHP showed high selectivity to NO over a number of reactive oxygen/nitrogen species (ROS/RNS) as well as high sensitivity (detection limit at 25 nM), pH stability and bio-compatibility. Furthermore, Mito-DHP proved to target mitochondria specifically and to visualize both exogenous and endogenous NO in real time.

引用

页码：382 / 388

页数：7

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