CRISPR/Cas9 system: a reliable and facile genome editing tool in modern biology

被引：22
作者：
Javaid, Darakhshan [1 ]
Ganie, Shahid Yousuf [1 ]
Hajam, Younis Ahmad [2 ]
Reshi, Mohd Salim [1 ]
机构：
[1] Baba Ghulam Shah Badshah Univ, Sch Biosci & Biotechnol, Dept Zool, Toxicol & Pharmacol Lab, Rajouri 185234, Jammu & Kashmir, India
[2] St Baba Bhag Singh Univ, Dept Life Sci & Allied Hlth Sci, Jalandhar 144030, Punjab, India
来源：
MOLECULAR BIOLOGY REPORTS | 2022年 / 49卷 / 12期
关键词：
CRISPR; Cas9; Genome editing; gRNA; Double strand break; Model animals; ONE-STEP GENERATION; IN-VIVO; TRANSCRIPTIONAL ACTIVATION; CYNOMOLGUS MONKEY; GENE REPRESSION; CARCINOMA CELLS; KNOCKOUT PIGS; RNA; DELIVERY; EFFICIENT;
D O I：
10.1007/s11033-022-07880-6
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Genome engineering has always been a versatile technique in biological research and medicine, with several applications. In the last several years, the discovery of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-Cas9 technology has swept the scientific community and revolutionised the speed of modern biology, heralding a new era of disease detection and rapid biotechnology discoveries. It enables successful gene editing by producing targeted double-strand breaks in virtually any organism or cell type. So, this review presents a comprehensive knowledge about the mechanism and structure of Cas9-mediated RNA-guided DNA targeting and cleavage. In addition, genome editing via CRISPR-Cas9 technology in various animals which are being used as models in scientific research including Non-Human Primates Pigs, Dogs, Zebra, fish and Drosophila has been discussed in this review. This review also aims to understand the applications, serious concerns and future perspective of CRISPR/Cas9-mediated genome editing.
引用
收藏
页码：12133 / 12150
页数：18
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