Nanostructured lipid carriers as a novel tool to deliver sclareol: physicochemical characterisation and evaluation in human cancer cell lines

被引：4

作者：

Marques Borges, Gabriel Silva ^{[1
]}

Dias Moura Prazeres, Pedro Henrique ^{[2
]}

de Souza, Angelo Malachias ^{[3
]}

Yoshida, Maria Irene ^{[4
]}

Carneiro Vilela, Jose Mario ^{[5
]}

Maciel e Silva, Aline Teixeira ^{[1
]}

Oliveira, Mariana Silva ^{[1
]}

Gomes, Dawidson Assis ^{[6
]}

Andrade, Margareth Spangler ^{[5
]}

de Souza-Fagundes, Elaine Maria ^{[2
]}

Miranda Ferreira, Lucas Antonio ^{[1
]}

机构：

[1] Univ Fed Minas Gerais, Fac Pharm, Dept Pharmaceut, Belo Horizonte, MG, Brazil

[2] Univ Fed Minas Gerais, Biol Sci Inst, Dept Physiol & Biophys, Belo Horizonte, MG, Brazil

[3] Univ Fed Minas Gerais, Inst Exact Sci, Dept Phys, Belo Horizonte, MG, Brazil

[4] Univ Fed Minas Gerais, Inst Exact Sci, Dept Chem, Belo Horizonte, MG, Brazil

[5] Technol & Innovat Ctr, Ind Learning Natl Serv, Belo Horizonte, MG, Brazil

[6] Univ Fed Minas Gerais, Biol Sci Inst, Dept Biochem & Immunol, Belo Horizonte, MG, Brazil

来源：

BRAZILIAN JOURNAL OF PHARMACEUTICAL SCIENCES | 2021年 / 57卷

关键词：

Cancer; Sclareol; Solid lipid nanoparticles; Nanostructured lipid carriers; Small-angle X-ray scattering; DRUG-DELIVERY; RETINOIC ACID; IN-VITRO; ANTICANCER ACTIVITY; BINARY-MIXTURES; TUMOR-GROWTH; APOPTOSIS; NANOPARTICLES; MECHANISM; IMPROVE;

D O I：

10.1590/s2175-97902020000418497

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Sclareol (SC) is arousing great interest due to its cytostatic and cytotoxic activities in several cancer cell lines. However, its hydrophobicity is a limiting factor for its in vivo administration. One way to solve this problem is through nanoencapsulation. Therefore, solid lipid nanoparticles (SLN-SC) and nanostructured lipid carriers (NLC-SC) loaded with SC were produced and compared regarding their physicochemical properties. NLC-SC showed better SC encapsulation than SLN-SC and was chosen to be compared with free SC in human cancer cell lines (MDA-MB-231 and HCT-116). Free SC had slightly higher cytotoxicity than NLC-SC and produced subdiploid DNA content in both cell lines. On the other hand, NLC-SC led to subdiploid content in MDA-MB-231 cells and G2/M checkpoint arrest in HCT-116 cells. These findings suggest that SC encapsulation in NLC is a way to allow the in vivo administration of SC and might alter its biological properties.

引用

页数：15

共 52 条

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