Nanostructured lipid carriers as a novel tool to deliver sclareol: physicochemical characterisation and evaluation in human cancer cell lines

被引：4
作者：
Marques Borges, Gabriel Silva [1 ]
Dias Moura Prazeres, Pedro Henrique [2 ]
de Souza, Angelo Malachias [3 ]
Yoshida, Maria Irene [4 ]
Carneiro Vilela, Jose Mario [5 ]
Maciel e Silva, Aline Teixeira [1 ]
Oliveira, Mariana Silva [1 ]
Gomes, Dawidson Assis [6 ]
Andrade, Margareth Spangler [5 ]
de Souza-Fagundes, Elaine Maria [2 ]
Miranda Ferreira, Lucas Antonio [1 ]
机构：
[1] Univ Fed Minas Gerais, Fac Pharm, Dept Pharmaceut, Belo Horizonte, MG, Brazil
[2] Univ Fed Minas Gerais, Biol Sci Inst, Dept Physiol & Biophys, Belo Horizonte, MG, Brazil
[3] Univ Fed Minas Gerais, Inst Exact Sci, Dept Phys, Belo Horizonte, MG, Brazil
[4] Univ Fed Minas Gerais, Inst Exact Sci, Dept Chem, Belo Horizonte, MG, Brazil
[5] Technol & Innovat Ctr, Ind Learning Natl Serv, Belo Horizonte, MG, Brazil
[6] Univ Fed Minas Gerais, Biol Sci Inst, Dept Biochem & Immunol, Belo Horizonte, MG, Brazil
来源：
BRAZILIAN JOURNAL OF PHARMACEUTICAL SCIENCES | 2021年 / 57卷
关键词：
Cancer; Sclareol; Solid lipid nanoparticles; Nanostructured lipid carriers; Small-angle X-ray scattering; DRUG-DELIVERY; RETINOIC ACID; IN-VITRO; ANTICANCER ACTIVITY; BINARY-MIXTURES; TUMOR-GROWTH; APOPTOSIS; NANOPARTICLES; MECHANISM; IMPROVE;
D O I：
10.1590/s2175-97902020000418497
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
Sclareol (SC) is arousing great interest due to its cytostatic and cytotoxic activities in several cancer cell lines. However, its hydrophobicity is a limiting factor for its in vivo administration. One way to solve this problem is through nanoencapsulation. Therefore, solid lipid nanoparticles (SLN-SC) and nanostructured lipid carriers (NLC-SC) loaded with SC were produced and compared regarding their physicochemical properties. NLC-SC showed better SC encapsulation than SLN-SC and was chosen to be compared with free SC in human cancer cell lines (MDA-MB-231 and HCT-116). Free SC had slightly higher cytotoxicity than NLC-SC and produced subdiploid DNA content in both cell lines. On the other hand, NLC-SC led to subdiploid content in MDA-MB-231 cells and G2/M checkpoint arrest in HCT-116 cells. These findings suggest that SC encapsulation in NLC is a way to allow the in vivo administration of SC and might alter its biological properties.
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页数：15
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