A Trypanosoma cruzi zinc finger protein that is implicated in the control of epimastigote-specific gene expression and metacyclogenesis

被引:8
作者
Tavares, Thais S. [1 ]
Mugge, Fernanda L. B. [1 ,2 ,3 ,4 ]
Grazielle-Silva, Viviane [1 ]
Valente, Bruna M. [1 ]
Goes, Wanessa M. [1 ]
Oliveira, Antonio E. R. [1 ]
Belew, Ashton T. [2 ,3 ]
Guarneri, Alessandra A. [5 ]
Pais, Fabiano S. [5 ]
El-Sayed, Najib M. [2 ,3 ]
Teixeira, Santuza M. R. [1 ]
机构
[1] Univ Fed Minas Gerais, Dept Bioquim & Imunol, BR-31270901 Belo Horizonte, MG, Brazil
[2] Univ Maryland, Dept Cell Biol & Mol Genet, College Pk, MD 20742 USA
[3] Univ Maryland, Ctr Bioinformat & Computat Biol, College Pk, MD 20742 USA
[4] Heidelberg Univ, Ctr Mol Biol, D-69120 Heidelberg, Germany
[5] Inst Rene Rachou, Fundacao Oswaldo Cruz, BR-30190009 Belo Horizonte, MG, Brazil
基金
英国科研创新办公室;
关键词
Parasite differentiation; post-transcriptional control; RNA binding proteins; transcriptome analyses; Trypanosoma cruzi; MESSENGER-RNAS; DIFFERENTIATION; SEQUENCE; BRUCEI; TCRBP19; TCPUF6;
D O I
10.1017/S0031182020002176
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
Trypanosoma cruzi has three biochemically and morphologically distinct developmental stages that are programmed to rapidly respond to environmental changes the parasite faces during its life cycle. Unlike other eukaryotes, Trypanosomatid genomes contain protein coding genes that are transcribed into polycistronic pre-mRNAs and have their expression controlled by post-transcriptional mechanisms. Transcriptome analyses comparing three stages of the T. cruzi life cycle revealed changes in gene expression that reflect the parasite adaptation to distinct environments. Several genes encoding RNA binding proteins (RBPs), known to act as key post-transcriptional regulatory factors, were also differentially expressed. We characterized one T. cruzi RBP, named TcZH3H12, which contains a zinc finger domain and is up-regulated in epimastigotes compared to trypomastigotes and amastigotes. TcZC3H12 knockout (KO) epimastigotes showed decreased growth rates and increased capacity to differentiate into metacyclic trypomastigotes. Transcriptome analyses comparing wild type and TcZC3H12 KOs revealed a TcZC3H12-dependent expression of epimastigote-specific genes such as genes encoding amino acid transporters and proteins associated with differentiation (PADs). RNA immunoprecipitation assays showed that transcripts from the PAD family interact with TcZC3H12. Taken together, these findings suggest that TcZC3H12 positively regulates the expression of genes involved in epimastigote proliferation and also acts as a negative regulator of metacyclogenesis.
引用
收藏
页码:1171 / 1185
页数:15
相关论文
共 71 条
  • [41] A novel CCCH protein which modulates differentiation of Trypanosoma brucei to its procyclic form
    Hendriks, EF
    Robinson, DR
    Hinkins, M
    Matthews, KR
    [J]. EMBO JOURNAL, 2001, 20 (23) : 6700 - 6711
  • [42] Protein and mRNA content of TcDHH1-containing mRNPs in Trypanosoma cruzi
    Holetz, Fabiola B.
    Alves, Lysangela R.
    Probst, Christian M.
    Dallagiovanna, Bruno
    Marchini, Fabricio K.
    Manque, Patricio
    Buck, Gregory
    Krieger, Marco A.
    Correa, Alejandro
    Goldenberg, Samuel
    [J]. FEBS JOURNAL, 2010, 277 (16) : 3415 - 3426
  • [43] Evidence of P-body-like structures in Trypanosoma cruzi
    Holetz, Fabiola Barbieri
    Correa, Alejandro
    Avila, Andrea Rodrigues
    Nakamura, Celso Vataru
    Krieger, Marco Aurelio
    Goldenberg, Samuel
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2007, 356 (04) : 1062 - 1067
  • [44] Distinct genomic organization, mRNA expression and cellular localization of members of two amastin sub-families present in Trypanosoma cruzi
    Kangussu-Marcolino, Monica Mendes
    Cardoso de Paiva, Rita Marcia
    Araujo, Patricia Rosa
    de Mendonca-Neto, Rondon Pessoa
    Lemos, Laiane
    Bartholomeu, Daniella Castanheira
    Mortara, Renato A.
    daRocha, Wanderson Duarte
    Ribeiro Teixeira, Santuza Maria
    [J]. BMC MICROBIOLOGY, 2013, 13
  • [45] Graph-based genome alignment and genotyping with HISAT2 and HISAT-genotype
    Kim, Daehwan
    Paggi, Joseph M.
    Park, Chanhee
    Bennett, Christopher
    Salzberg, Steven L.
    [J]. NATURE BIOTECHNOLOGY, 2019, 37 (08) : 907 - +
  • [46] Genome-wide in silico screen for CCCH-type zinc finger proteins of Trypanosoma brucei, Trypanosoma cruzi and Leishmania major
    Kramer, Susanne
    Kimblin, Nicola C.
    Carrington, Mark
    [J]. BMC GENOMICS, 2010, 11
  • [47] The sva package for removing batch effects and other unwanted variation in high-throughput experiments
    Leek, Jeffrey T.
    Johnson, W. Evan
    Parker, Hilary S.
    Jaffe, Andrew E.
    Storey, John D.
    [J]. BIOINFORMATICS, 2012, 28 (06) : 882 - 883
  • [48] Fast and accurate short read alignment with Burrows-Wheeler transform
    Li, Heng
    Durbin, Richard
    [J]. BIOINFORMATICS, 2009, 25 (14) : 1754 - 1760
  • [49] Transcriptome Remodeling in Trypanosoma cruzi and Human Cells during Intracellular Infection
    Li, Yuan
    Shah-Simpson, Sheena
    Okrah, Kwame
    Belew, A. Trey
    Choi, Jungmin
    Caradonna, Kacey L.
    Padmanabhan, Prasad
    Ndegwa, David M.
    Temanni, M. Ramzi
    Bravo, Hector Corrada
    El-Sayed, Najib M.
    Burleigh, Barbara A.
    [J]. PLOS PATHOGENS, 2016, 12 (04)
  • [50] The zinc finger proteins ZC3H20 and ZC3H21 stabilise mRNAs encoding membrane proteins and mitochondrial proteins in insect-form Trypanosoma brucei
    Liu, Bin
    Marucha, Kevin Kamanyi
    Clayton, Christine
    [J]. MOLECULAR MICROBIOLOGY, 2020, 113 (02) : 430 - 451