The structure of the first representative of Pfam family PF06475 reveals a new fold with possible involvement in glycolipid metabolism

被引：7

作者：

Bakolitsa, Constantina ^{[1
]}

Kumar, Abhinav ^{[2
]}

McMullan, Daniel ^{[3
]}

Krishna, S. Sri ^{[1
,4
]}

Miller, Mitchell D. ^{[2
]}

Carlton, Dennis ^{[5
]}

Najmanovich, Rafael ^{[6
]}

Abdubek, Polat ^{[3
]}

Astakhova, Tamara ^{[4
]}

Chiu, Hsiu-Ju ^{[2
]}

Clayton, Thomas ^{[5
]}

Deller, Marc C. ^{[5
]}

Duan, Lian ^{[4
]}

Elias, Ylva ^{[5
]}

Feuerhelm, Julie ^{[3
]}

Grant, Joanna C. ^{[3
]}

Grzechnik, Slawomir K. ^{[4
]}

Han, Gye Won ^{[5
]}

Jaroszewski, Lukasz ^{[1
,4
]}

Jin, Kevin K. ^{[2
]}

Klock, Heath E. ^{[3
]}

Knuth, Mark W. ^{[3
]}

Kozbial, Piotr ^{[1
]}

Marciano, David ^{[5
]}

Morse, Andrew T. ^{[4
]}

Nigoghossian, Edward ^{[3
]}

Okach, Linda ^{[3
]}

Oommachen, Silvya ^{[2
]}

Paulsen, Jessica ^{[3
]}

Reyes, Ron ^{[2
]}

Rife, Christopher L. ^{[2
]}

Trout, Christina V. ^{[5
]}

van den Bedem, Henry ^{[2
]}

Weekes, Dana ^{[1
]}

White, Aprilfawn ^{[3
]}

Xu, Qingping ^{[2
]}

Hodgson, Keith O.

Wooley, John ^{[4
]}

Elsliger, Marc-Andre ^{[5
]}

Deacon, Ashley M. ^{[2
]}

Godzik, Adam ^{[1
,4
]}

Lesley, Scott A. ^{[3
,5
]}

Wilson, Ian A. ^{[5
]}

机构：

[1] Burnham Inst Med Res, Program Bioinformat & Syst Biol, La Jolla, CA USA

[2] SLAC Natl Accelerator Lab, Stanford Synchrotron Radiat Lightsource, Menlo Pk, CA USA

[3] Novartis Res Fdn, Genom Inst, Prot Sci Dept, San Diego, CA USA

[4] Univ Calif San Diego, Ctr Res Biol Syst, La Jolla, CA 92093 USA

[5] Scripps Res Inst, Dept Mol Biol, La Jolla, CA 92037 USA

[6] Univ Sherbrooke, Dept Biochim, Quebec City, PQ, Canada

来源：

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2010年 / 66卷

基金：

美国国家卫生研究院;

关键词：

PROTEIN-STRUCTURE; CELL-WALL; ANGSTROM RESOLUTION; HYDROPHOBIC CAVITY; CRYSTAL-STRUCTURE; BINDING; LOLA; LIPOPOLYSACCHARIDE; DEHYDROGENASE; BIOSYNTHESIS;

D O I：

10.1107/S1744309109022684

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The crystal structure of PA1994 from Pseudomonas aeruginosa, a member of the Pfam PF06475 family classified as a domain of unknown function (DUF1089), reveals a novel fold comprising a 15-stranded beta-sheet wrapped around a single alpha-helix that assembles into a tight dimeric arrangement. The remote structural similarity to lipoprotein localization factors, in addition to the presence of an acidic pocket that is conserved in DUF1089 homologs, phospholipid-binding and sugar-binding proteins, indicate a role for PA1994 and the DUF1089 family in glycolipid metabolism. Genome-context analysis lends further support to the involvement of this family of proteins in glycolipid metabolism and indicates possible activation of DUF1089 homologs under conditions of bacterial cell-wall stress or host-pathogen interactions.

引用

页码：1211 / 1217

页数：7

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