Lactose biosensor based on lactase and galactose oxidase immobilized in polyvinyl formal

被引：12
作者：
Sharma, Sandeep K.
Kumar, Ashok
Chaudhary, Reeti
Pundir, Suman
Pundir, C. S.
Sehgal, Neeta
机构：
[1] Inst Genom & Integrat Biol, Delhi 110007, India
[2] Maharshi Dayanand Univ, Dept Biochem & Genet, Rohtak, Haryana, India
[3] Univ Delhi, Dept Zool, Delhi 110007, India
来源：
ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY | 2007年 / 35卷 / 04期
关键词：
galactose oxidase; beta-galactosidase; lactase; lactose biosensor; lactose intolerance; polyvinyl formal;
D O I：
10.1080/10731190701460309
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A lactose biosensor was developed by immobilizing lactase and galactose oxidase in a polyvinyl formal membrane and was attached to the oxygen electrode of a dissolved oxygen analyzer for estimation of lactose in milk and food products. The enzyme immobilized polyvinyl formal membrane was characterized by atomic force microscopy. The biosensor showed the linearity for 1-7 g dl(-1) of lactose and can be reused for up to 20 measurements. The effects of pH, temperature and the stability of the immobilized lactase and galactose oxidase in PVF membrane were also studied. The enzyme membrane was found stable up to 35 degrees C and had a shelf-life of more than three months at 4 degrees C.
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页码：421 / 430
页数：10
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