A non-isotopic method for the detection of telomerase activity in tumour tissues: TRAP-silver staining assay

被引：26
|
作者：
Wen, JM [1 ]
Sun, LB
Zhang, M
Zheng, MH
机构：
[1] Sun Yat Sen Univ Med Sci, Dept Pathol, Guangzhou 510089, Peoples R China
[2] Univ Western Australia, Dept Orthopaed Surg, Nedlands, WA 6009, Australia
来源：
JOURNAL OF CLINICAL PATHOLOGY-MOLECULAR PATHOLOGY | 1998年 / 51卷 / 02期
关键词：
telomerase; TRAP; bone tumours; silver staining;
D O I：
10.1136/mp.51.2.110
中图分类号：
R36 [病理学];
学科分类号：
100104 ;
摘要：
Telomerase activity has been observed in a high proportion of specimens from a wide variety of human malignant tumours, but not in non-neoplastic tissues. Measurement of telomerase activity is a possible diagnostic maker for malignant tumours. A non-isotopic telomeric repeat amplification protocol (TRAP) silver staining method for the detection of telomerase activity in human bone tumour tissues is described. The TRAP-silver staining method was quick, safe, and effective, and can be used as a routine diagnostic method for the detection of telomerase activity in bone tumours.
引用
收藏
页码：110 / 112
页数：3
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