Kinetics of Ergothioneine Inhibition of Mushroom Tyrosinase

被引：27

作者：

Liao, Wayne C. ^{[2
]}

Wu, Wen Hong ^{[3
]}

Tsai, Pei-Chuan ^{[1
]}

Wang, Hui-Feng ^{[1
]}

Liu, Yi-Hsin ^{[1
]}

Chan, Chin-Feng ^{[1
]}

机构：

[1] Hungkuang Univ, Dept Appl Cosmetol, Taichung 433, Taiwan

[2] Chang Gung Inst Technol, Dept Nursing, Chiayi, Taiwan

[3] Kuang Tien Gen Hosp, Plast & Reconstruct Cosmet Surg Ctr, Taichung, Taiwan

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 2012年 / 166卷 / 02期

关键词：

Ergothioneine; Histidine; Tyrosinase; DPPH; OXIDATION; MECHANISM;

D O I：

10.1007/s12010-011-9421-x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The native amino acid ergothioneine, a thiourea derivative of histidine, inhibits mushroom tyrosinase activity in a dose-dependent manner, with an IC50 value of 1.025 mg/ml (4.47 mM). By contrast, histidine exhibited no inhibitory effect on mushroom tyrosinase activity. We characterized ergothioneine as a noncompetitive tyrosinase inhibitor using a Lineweaver-Burk plot of experimental kinetic data. The IC50 value for ergothioneine scavenging of 2,2-diphenyl-1-picrylhydrazyl was 6.110+/-0.305 mg/ml, much higher than the IC50 for inhibition of tyrosinase activity which indicating ergothioneine on tyrosinase shows a weak correlation to its antioxidative activity. The results demonstrated that ergothioneine has a potent inhibition effect on tyrosinase enzyme activity, resulting from the presence of the sulfur substituted imidazole ring in ergothioneine.

引用

页码：259 / 267

页数：9

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