Transcription of promoter from the human APRIL gene regulated by Sp1 and NF-kB

被引：10

作者：

Xu, J. ^{[1
]}

Ding, W. F. ^{[1
]}

Shao, K. K. ^{[1
]}

Wang, X. D. ^{[1
]}

Wang, G. H. ^{[1
]}

Li, H. Q. ^{[1
]}

Wang, H. M. ^{[1
]}

机构：

[1] Nantong Univ, Dept Clin Lab Ctr, Affiliated Hosp, Nantong 266001, Peoples R China

来源：

NEOPLASMA | 2012年 / 59卷 / 03期

关键词：

APRIL; promoter; Luciferase reporter assay; Sp1; NF-kB; NECROSIS-FACTOR FAMILY; COLON-CARCINOMA CELLS; FACTOR-KAPPA-B; EXPRESSION; RECEPTOR; CANCER; ACTIVATION; PROTEINS; INSIGHTS; BINDING;

D O I：

10.4149/neo_2012_044

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

A proliferation-inducing ligand (APRIL) which stimulates the cell proliferation is abundantly expressed in colorectal cancer (CRC) tumors. In this report, the promoter region of the APRIL gene was determined and the major transcription factor was investigated for the first time. Deletion analysis of 5'-flanking region of the human APRIL gene and transient transfection revealed that a 538 bp region (from -1539 to -1001) was essential for promoter activation of the APRIL gene. The data from electrophoretic mobility shift assays (EMSA) indicated that the 538 bp promoter region was responsive to the specificity protein 1 (Sp1) and nuclear factor kappa B (NF-kB). Overexpression of Sp1 or NF-kB increased the activity of the APRIL promoter. Mithramycin A (inhibitor of Sp1) and Bay11-7082 (inhibitor of NF-kB) exhibited an inhibitory activity to APRIL promoter. Our results will benefit to the APRIL gene regulation investigation and contribute to discover new drug target for the APRIL gene therapy of CRC.

引用

页码：341 / 347

页数：7

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