Metallic wear debris may regulate CXCR4 expression in vitro and in vivo

被引:14
作者
Drynda, Andreas [1 ]
Singh, Gurpal [1 ,2 ]
Buchhorn, Gottfried H. [3 ]
Awiszus, Friedemann [1 ]
Ruetschi, Marcel [4 ]
Feuerstein, Bernd [5 ]
Kliche, Stefanie [6 ]
Lohmann, Christoph H. [1 ]
机构
[1] Univ Magdeburg, Dept Orthopaed Surg, D-39106 Magdeburg, Germany
[2] Natl Univ Hlth Syst, Univ Orthopaed Hand & Reconstruct Microsurg Clust, Singapore, Singapore
[3] Univ Med Ctr, Dept Orthopaed, Gottingen, Germany
[4] Loretto Hosp, Dept Orthopaed Surg, Freiburg, Germany
[5] Magdeburg Stendal Univ Appl Sci, Dept Mech Engn, Magdeburg, Germany
[6] Univ Magdeburg, Inst Mol & Clin Immunol, D-39106 Magdeburg, Germany
关键词
chemokine receptors; metallic debris; orthopedic implants; cobaltchromium alloys; NF-KAPPA-B; HUMAN-IMMUNODEFICIENCY-VIRUS; TOTAL HIP-ARTHROPLASTY; OSTEOCLAST PRECURSORS; FAILED METAL; PIVOTAL ROLE; BEARINGS; RECRUITMENT; PROMOTES; RELEASE;
D O I
10.1002/jbm.a.35330
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
CXCR4, the chemokine receptor for CXCL12, also known as SDF-1 (stromal cell derived factor-1), has been shown to play a pivotal role in bone metastasis, inflammatory, and autoimmune conditions but has not been investigated in periprosthetic osteolysis. We co-cultured osteoblast-like cells with increasing concentrations of metallic (Co-35Ni-20Cr-10Mo and Co-28Cr-6Mo) and Co-ions simulating wear debris. Real-time polymerase chain reaction (RT-PCR) and Western blotting were used to quantify gene and protein expression of CXCR4. The expression of tumor necrosis factor-alpha (TNF-) and the effects of AMD3100 (bicyclam) on both CXCR4 and TNF- expression among these cells was investigated. RT-PCR showed an increase in CXCR4 mRNA (7.5-fold for MG63 and 4.0-fold for SaOs-2 cells) among cells co-cultured with metal alloy particles. Western blotting showed a time-dependent increase in protein expression of CXCR4. The attempted blockade of CXCR4 by its known competitive receptor agonist AMD3100 led to a significant inhibition TNF- mRNA expression. Immunohistochemistry showed CXCR4 positivity among patients with failed metal-on-metal hip replacements and radiographic evidence of osteolysis. Our data collectively suggest that the CXCR4 chemokine is upregulated in a dose- and time-dependent manner in the presence of metallic wear debris. (c) 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 1940-1948, 2015.
引用
收藏
页码:1940 / 1948
页数:9
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