A mild extraction and separation procedure of polysaccharide, lipid, chlorophyll and protein from Chlorella spp.

被引：36

作者：

Zhao, Wenhuan ^{[1
]}

Duan, Meirong ^{[1
]}

Zhang, Xu ^{[1
]}

Tan, Tianwei ^{[1
]}

机构：

[1] Beijing Univ Chem Technol, Coll Life Sci & Technol, Natl Energy R&D Ctr Biorefinery, Beijing 100029, Peoples R China

来源：

RENEWABLE ENERGY | 2018年 / 118卷

关键词：

Chlorella spp; Polysaccharides; Lipid; Protein; Chlorophyll; AQUEOUS 2-PHASE EXTRACTION; MICROALGAE CHLORELLA; COPPER CHLOROPHYLLIN; BIODIESEL; PURIFICATION; HYDROLYSIS; DISRUPTION;

D O I：

10.1016/j.renene.2017.11.046

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

This study developed a way to separate lipid, polysaccharide, protein and chlorophyll from Wet Chlorella spp. (85% moisture content, wt) by using three-phase partitioning (TPP). The optimum conditions for the key steps were researched and defined as (I) cell disruption at the following conditions: biomass concentration 20 g/L, bath temperature 90 degrees C, duration 2.5 h, enzyme concentration 12 g/L, enzyme hydrolysis time 4 h; and (II) TPP at 40% (NH4)(2)SO4 (wt, %), sample solution/t-butanol 1: 2 (V/V), room temperature. The extraction yield of lipid and the poysaccharide preservation rate by the optimized procedure were 80.24%, 90.63%, respectively. The protein removal rate was 78.87%. The results provided a promising towards the industrial separation of lipid, polysaccaride, protein and chlorophyll. (C) 2017 Elsevier Ltd. All rights reserved.

引用

页码：701 / 708

页数：8

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