Detection of an anhydride intermediate in the carboxypeptidase a catalyzed hydrolysis of a peptide substrate by solid state NMR spectroscopy and its mechanistic implication

被引：14

作者：

Lee, HC

Ko, YH

Bin Baek, S

Kim, DH

机构：

[1] Pohang Univ Sci & Technol, Dept Chem, Pohang 790784, South Korea

[2] Pohang Univ Sci & Technol, Ctr Biofunct Mol, Pohang 790784, South Korea

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 1998年 / 8卷 / 23期

关键词：

D O I：

10.1016/S0960-894X(98)00624-6

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

We have detected an anhydride intermediate in the CPA catalyzed proteolytic reaction of Gly-Tyr. It appears that since the zinc-bound water molecule which is believed to attack the scissile amide carbonyl carbon in the hydrolysis reaction is excluded by the N-terminal amino group of Gly-Tyr, the carboxylate of Glu-270 becomes to attack the amide bond to generate the anhydride intermediate. (C) 1998 Elsevier Science Ltd. All rights reserved.

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页码：3379 / 3384

页数：6

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