Evaluation of Sperm Proteins in Infertile Men: A Proteomic Approach

被引：56

作者：

Thacker, Stetson ^{[2
]}

Yadav, Satya P. ^{[2
]}

Sharma, Rakesh K. ^{[1
,3
]}

Kashou, Anthony ^{[1
,3
]}

Willard, Belinda ^{[4
]}

Zhang, Dongmei ^{[4
]}

Agarwal, Ashok ^{[1
,3
]}

机构：

[1] Cleveland Clin, Glickman Urol & Kidney Inst, Ctr Reprod Med, Cleveland, OH 44195 USA

[2] Cleveland Clin, Lerner Res Inst, Mol Biotechnol Core Lab, Cleveland, OH 44195 USA

[3] Womens Hlth Inst, Cleveland, OH USA

[4] Cleveland Clin Fdn, Lerner Res Inst, Cleveland, OH 44195 USA

来源：

FERTILITY AND STERILITY | 2011年 / 95卷 / 08期

关键词：

Sperm; semenogelin II precursor; prolactin-induced protein; clusterin; prostate-specific antigen; PROSTATE-SPECIFIC ANTIGEN; SULFATED GLYCOPROTEIN-2; SEMINAL PLASMA; CLUSTERIN; MOTILITY; IDENTIFICATION; SPERMATOZOA; COMPLEMENT; CELLS; SUBSTRATE;

D O I：

10.1016/j.fertnstert.2011.03.112

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

In this study, the sperm protein profile was compared between fertile and infertile men using 2-dimensional gel electrophoresis, liquid chromatography mass spectrometer analysis, and matrix-assisted laser desorption ionization-time-of-flight-mass spectrometry. Four unique proteins, semenogelin II precursor, prolactin-induced protein, clusterin isoform 1, and prostate-specific antigen isoform 1 preproprotein, were predominantly present in the semen of healthy men; however, semenogelin II precursor and clusterin isoform 1 were not seen in the semen of infertile men, suggesting unique differences in the spermatozoa protein profiles of fertile and infertile men. (Fertil Steril (R) 2011;95:2745-8. (C)2011 by American Society for Reproductive Medicine.)

引用

页码：2745 / 2748

页数：4

共 40 条

[1]

Ahlgren G, 1995, J ANDROL, V16, P491

[2]

[Anonymous], DIS BREAST

[3] INTERACTION OF SEMINAL PLASMA-PROTEINS WITH CELL-SURFACE ANTIGENS - PRESENCE OF A CD4-BINDING GLYCOPROTEIN IN HUMAN SEMINAL PLASMA [J].

AUTIERO, M ;

ABRESCIA, P ;

GUARDIOLA, J .

EXPERIMENTAL CELL RESEARCH, 1991, 197 (02) :268-271

[4] Multiple receptors mediate apoJ-dependent clearance of cellular debris into nonprofessional phagocytes [J].

Bartl, MM ;

Luckenbach, T ;

Bergner, O ;

Ullrich, O ;

Koch-Brandt, C .

EXPERIMENTAL CELL RESEARCH, 2001, 271 (01) :130-141

[5] CD4-mediated anchoring of the seminal antigen gp17 onto the spermatozoon surface [J].

Bergamo, P ;

Balestrieri, M ;

Cammarota, G ;

Guardiola, J ;

Abrescia, P .

HUMAN IMMUNOLOGY, 1997, 58 (01) :30-41

[6] INDUCTION OF THE TRPM-2 GENE IN CELLS UNDERGOING PROGRAMMED DEATH [J].

BUTTYAN, R ;

OLSSON, CA ;

PINTAR, J ;

CHANG, CS ;

BANDYK, M ;

NG, PY ;

SAWCZUK, IS .

MOLECULAR AND CELLULAR BIOLOGY, 1989, 9 (08) :3473-3481

[7] Biosynthesis and immunobiochemical characterization of gp17/GCDFP-15 -: A glycoprotein from seminal vesicles and from breast tumors, in HeLa cells and in Pichia pastoris yeast [J].

Caputo, E ;

Carratore, V ;

Ciullo, M ;

Tiberio, C ;

Mani, JC ;

Piatier-Tonneau, D ;

Guardiola, J .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1999, 265 (02) :664-670

[8] A novel aspartyl proteinase from apocrine epithelia and breast tumors [J].

Caputo, E ;

Manco, G ;

Mandrich, L ;

Guardiola, J .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (11) :7935-7941

[9]

Clark AM, 1997, J ANDROL, V18, P257

[10] BIOSYNTHESIS AND MOLECULAR-CLONING OF SULFATED GLYCOPROTEIN-2 SECRETED BY RAT SERTOLI CELLS [J].

COLLARD, MW ;

GRISWOLD, MD .

BIOCHEMISTRY, 1987, 26 (12) :3297-3303

← 1 2 3 4 →