Cytoskeletal forces during signaling activation in Jurkat T-cells

被引:122
|
作者
Hui, King Lam [1 ]
Balagopalan, Lakshmi [3 ]
Samelson, Lawrence E. [3 ]
Upadhyaya, Arpita [1 ,2 ]
机构
[1] Univ Maryland, Dept Phys, College Pk, MD 20742 USA
[2] Univ Maryland, Inst Phys Sci & Technol, College Pk, MD 20742 USA
[3] NCI, Cellular & Mol Biol Lab, Ctr Canc Res, NIH, Bethesda, MD 20892 USA
基金
美国国家科学基金会; 美国国家卫生研究院;
关键词
MYOSIN-II FUNCTION; IMMUNOLOGICAL SYNAPSE; TRACTION FORCES; ARP2/3; COMPLEX; RECEPTOR MICROCLUSTERS; ACTIN CYTOSKELETON; RETROGRADE FLOW; SUBSTRATE; RIGIDITY; MOTILITY;
D O I
10.1091/mbc.E14-03-0830
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
T-cells are critical for the adaptive immune response in the body. The binding of the T-cell receptor (TCR) with antigen on the surface of antigen-presenting cells leads to cell spreading and signaling activation. The underlying mechanism of signaling activation is not completely understood. Although cytoskeletal forces have been implicated in this process, the contribution of different cytoskeletal components and their spatial organization are unknown. Here we use traction force microscopy to measure the forces exerted by Jurkat T-cells during TCR activation. Perturbation experiments reveal that these forces are largely due to actin assembly and dynamics, with myosin contractility contributing to the development of force but not its maintenance. We find that Jurkat T-cells are mechanosensitive, with cytoskeletal forces and signaling dynamics both sensitive to the stiffness of the substrate. Our results delineate the cytoskeletal contributions to interfacial forces exerted by T-cells during activation.
引用
收藏
页码:685 / 695
页数:11
相关论文
共 50 条
  • [1] Cytoskeletal forces in Jurkat T cells contribute to signaling activation
    Hui, K.
    Upadhyaya, A.
    MOLECULAR BIOLOGY OF THE CELL, 2013, 24
  • [2] Cytoskeletal Forces during T Cell Activation
    Hui, King Lam
    Upadhyaya, Arpita
    BIOPHYSICAL JOURNAL, 2015, 108 (02) : 142A - 142A
  • [3] Cytoskeletal forces during T cell activation.
    Hui, K.
    Upadhyaya, A.
    MOLECULAR BIOLOGY OF THE CELL, 2014, 25
  • [4] Comparative microarray analysis of gene expression during activation of human peripheral blood T-cells and leukemic Jurkat T-cells
    Um, TH
    Lin, Z
    Fillmore, CG
    Elenitoba-Johnson, KSJ
    Lim, MS
    MODERN PATHOLOGY, 2002, 15 (01) : 308A - 308A
  • [5] Comparative microarray analysis of gene expression during activation of human peripheral blood T-cells and leukemic Jurkat T-cells
    Um, TH
    Lin, Z
    Fillmore, CG
    Elenitoba-Johnson, KSJ
    Lim, MS
    LABORATORY INVESTIGATION, 2002, 82 (01) : 308A - 308A
  • [6] Comparative microarray analysis of gene expression during activation of human peripheral blood T-cells and leukemic Jurkat T-cells.
    Lin, ZS
    Fillmore, CG
    Elenitoba-Johnson, KSJ
    Lim, MS
    BLOOD, 2001, 98 (11) : 238A - 238A
  • [7] TYROSINE KINASE REGULATES CA2+ CURRENT ACTIVATION IN JURKAT T-CELLS
    CHUNG, SC
    GARDNER, P
    JOURNAL OF CELLULAR BIOCHEMISTRY, 1994, : 397 - 397
  • [8] Insolubility of T cell receptors in human Jurkat T-Cells
    Marano, Nadia
    Mutoru, Jane
    Glazier, Samantha
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2006, 231
  • [9] CD28 SIGNALING IN THE ACTIVATION OF NAIVE T-CELLS
    LASSILA, O
    VAINIO, O
    JOURNAL OF CELLULAR BIOCHEMISTRY, 1994, : 430 - 430
  • [10] Potential Effect of β-Estradiol in Human Jurkat T-Cells
    Hullitt, JoAnna
    Davis, Corey
    Yedjou, Clement
    Cameron, Joseph A.
    FASEB JOURNAL, 2009, 23