NmDef02, a novel antimicrobial gene isolated from Nicotiana megalosiphon confers high-level pathogen resistance under greenhouse and field conditions

被引：69

作者：

Portieles, Roxana ^{[1
]}

Ayra, Camilo ^{[1
]}

Gonzalez, Ernesto ^{[1
]}

Gallo, Araiz ^{[1
]}

Rodriguez, Raisa ^{[1
]}

Chacon, Osmany ^{[2
]}

Lopez, Yunior ^{[1
]}

Rodriguez, Mayra ^{[1
]}

Castillo, Juan ^{[3
]}

Pujol, Merardo ^{[1
]}

Enriquez, Gil ^{[1
]}

Borroto, Carlos ^{[1
]}

Trujillo, Luis ^{[1
]}

Thomma, Bart P. H. J. ^{[4
]}

Borras-Hidalgo, Orlando ^{[1
]}

机构：

[1] Ctr Genet Engn & Biotechnol, Havana, Cuba

[2] Tobacco Res Inst, Havana, Cuba

[3] INCA, Havana, Cuba

[4] Wageningen Univ, Phytopathol Lab, Wageningen, Netherlands

来源：

PLANT BIOTECHNOLOGY JOURNAL | 2010年 / 8卷 / 06期

关键词：

plant defensin; recombinant proteins; transgenic plants; disease control; PLANT DEFENSIN; ANTIFUNGAL PROTEINS; EXPRESSION; ARABIDOPSIS; POTATO; RADISH; COMPONENTS; TOBACCO; PEPPER; FAMILY;

D O I：

10.1111/j.1467-7652.2010.00501.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

P>Plant defensins are small cysteine-rich peptides that inhibit the growth of a broad range of microbes. In this article, we describe NmDef02, a novel cDNA encoding a putative defensin isolated from Nicotiana megalosiphon upon inoculation with the tobacco blue mould pathogen Peronospora hyoscyami f.sp. tabacina. NmDef02 was heterologously expressed in the yeast Pichia pastoris, and the purified recombinant protein was found to display antimicrobial activity in vitro against important plant pathogens. Constitutive expression of NmDef02 gene in transgenic tobacco and potato plants enhanced resistance against various plant microbial pathogens, including the oomycete Phytophthora infestans, causal agent of the economically important potato late blight disease, under greenhouse and field conditions.

引用

页码：678 / 690

页数：13

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