Label-free detection of T4 DNA ligase and polynucleotide kinase activity based on toehold-mediated strand displacement and split G-quadruplex probes

被引：40

作者：

Guo, Yahui ^{[1
,2
]}

Wang, Qinglin ^{[2
]}

Wang, Zhili ^{[2
]}

Chen, Xing ^{[2
]}

Xu, Lijun ^{[2
]}

Hu, Jiming ^{[1
]}

Pei, Renjun ^{[2
]}

机构：

[1] Wuhan Univ, Coll Chem & Mol Sci, Minist Educ, Key Lab Analyt Chem Biol & Med, Wuhan, Peoples R China

[2] Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Div Nanobiomed, Key Lab Nanobio Interface, Suzhou 215123, Peoples R China

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2015年 / 214卷

基金：

中国国家自然科学基金;

关键词：

DNA ligase; Polynucleotide kinase; Label free; Toehold-mediated strand displacement; Split G-quadruplex; ROLLING CIRCLE AMPLIFICATION; LAMBDA EXONUCLEASE CLEAVAGE; AMPLIFIED DETECTION; ELECTROCHEMICAL DETECTION; CASCADE AMPLIFICATION; NUCLEIC-ACID; FLUORESCENCE; INHIBITION; LIGATION; BIOSENSOR;

D O I：

10.1016/j.snb.2015.03.013

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Herein, an efficient pair of split G-quadruplex probes was selected (S/N= 21) to construct a simple, rapid and label-free technique for the detections of T4 DNA ligase and PNK activity. DNA ligase and PNK are engaged in DNA replication and repair, ensuring the genetic stability. The detections of T4 ligase and PNK activity are of great significance in biology and biomedicine. Split G-quadruplex probes are robust label-free biosensing tools with turn-on signal readout. The selected pair of split G-quadruplex probes combined with toehold-mediated strand displacement in this work demonstrated an efficient strategy for monitoring the activities of T4 DNA ligase and PNK with good sensitivity. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：50 / 55

页数：6

共 53 条

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