Distribution And Occurrence of Root-Knot Nematodes (Meloidogyne spp.) in Kiwi Orchards in Western Black Sea Region

Objective of this study was to determine the prevalence and density of root-knot nematodes in kiwi orchards in the Western Black Sea Region. For this purpose. root and soil samples were taken from eight kiwi orchards in Duzce (2). Zonguldak (3), and Bartin (3) provinces in 2015 and 2016. Genomic DNA of the nematodes was extracted from second stage juviniles (J2), and :lea rDetections's real-time primers were used for identification of At tropical spp., M. hapla and M. enterelobii and specific universal ME / MR primers were used for molecular identifications of Meloidogyne spp. As a result of the study. 50% of the orchards were infected wit h Meloidogyne spp. It was determined that one orchard from each of Bart in and Zonguldak were infected with M. incognita. one orchard from Zonguldak with M. hapla and one orchard in Duzce with both M. incognita and M. hapla. It was determined that the population density in the orchards ranged between 31-252 J2/100 g soil and the highest. population density was found as 252 J2/100 g soil in august in Yanaz Village of Bartin.