Long non-coding RNA ELF3-antisense RNA 1 promotes osteosarcoma cell proliferation by upregulating Kruppel-like factor 12 potentially via methylation of the microRNA-205 gene

被引：10
作者：
Yuan, Jianmin [1 ]
Kang, Jianhui [2 ]
Yang, Min [1 ]
机构：
[1] Wannan Med Coll, Affiliated Hosp 1, Dept Orthoped & Traumatol, Wuhu 241001, Anhui, Peoples R China
[2] Wannan Med Coll, Affiliated Hosp 1, Dept Cardiol, 2 Zheshan West Rd, Wuhu 241001, Anhui, Peoples R China
来源：
ONCOLOGY LETTERS | 2020年 / 19卷 / 03期
关键词：
osteosarcoma; Kruppel-like factor 12; long non-coding RNA ELF3-antisense RNA 1; microRNA-205; LNCRNA;
D O I：
10.3892/ol.2020.11312
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
A recent study characterized the long non-coding RNA (lncRNA) ELF3-antisense RNA 1 (ELF3-AS1) as an oncogenic lncRNA in bladder cancer. The present study aimed to investigate the role of ELF3-AS1 in osteosarcoma (OS). It was found that ELF3-AS1 was upregulated in OS tissues, and ELF3-AS1 expression level increased with increasing clinical stage. In OS tissues, Kruppel-like factor 12 (KLF12) was positively correlated with ELF3-AS1, while microRNA (miR)-205 was negatively correlated with ELF3-AS1. ELF3-AS1 overexpression resulted in the upregulation of KLF12, but the downregulation of miR-205. Overexpression of miR-205 caused downregulation of KLF12, but had no significant effects on ELF3-AS1 expression. Overexpression of KLF12 showed no significant impact on ELF3-AS1 and miR-205. ELF3-AS1 and KLF12 overexpression resulted in an increased proliferation rate in OS cells, while miR-205 played an opposite role and attenuated the effects of ELF3-AS1 overexpression. ELF3-AS1 overexpression promoted the methylation of the miR-205 gene. Therefore, ELF3-AS1 may promote OS cell proliferation by upregulating KLF12 through the methylation of the miR-205 gene.
引用
收藏
页码：2475 / 2480
页数：6
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