Predicted spatial resolution of super-resolving fluorescence microscopy using two-color fluorescence dip spectroscopy

被引：16

作者：

Iketaki, Y

Watanabe, T

Sakai, SIM

Omatsu, T

Yamamoto, K

Fujii, M

机构：

[1] Olympus Opt Co Ltd, Hachioji, Tokyo 1928512, Japan

[2] Inst Mol Sci, Okazaki, Aichi 4448585, Japan

[3] Chiba Univ, Dept Engn, Inage Ku, Chiba 2630022, Japan

[4] Keio Univ, Dept Chem, Kohoku Ku, Yokohama, Kanagawa 2238522, Japan

来源：

APPLIED SPECTROSCOPY | 2003年 / 57卷 / 10期

关键词：

two-color fluorescence dip spectroscopy; first-order bessel beam; super resolving; fluorescence microscopy; rhodamine; 6G; fluorescence depletion process;

D O I：

10.1366/000370203769699234

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

The theoretical spatial resolution of the novel superposition fluorescence microscopy was investigated. This microscopy was based on the fluorescence depletion process observed by two-color fluorescence dip spectroscopy. The investigation showed that the microscopy gives a spatial resolution better than 100 nm using a nanosecond laser with a pulse energy of ∼nJ/pulse.

引用

页码：1312 / 1316

页数：5