Transport of glutathione S-conjugates in the yeasts Saccharomyces cerevisiae

被引：13

作者：

Zadzinski, R ^{[1
]}

Maszewski, J ^{[1
]}

Bartosz, G ^{[1
]}

机构：

[1] UNIV LODZ,DEPT CYTOPHYSIOL,PL-90237 LODZ,POLAND

来源：

CELL BIOLOGY INTERNATIONAL | 1996年 / 20卷 / 05期

关键词：

yeasts; Saccharomyces cerevisiae; glutathione S-conjugates; membrane; transport;

D O I：

10.1006/cbir.1996.0035

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Transport of 2,4-dinitrophenyl-S-glutathione (DNP-SG) and a fluorescent glutathione S-conjugate, bimane-S-glutathione (B-SG) was studied in the baker's yeasts (S. cerevisiae). Both conjugates were exported from the cells; the transport was inhibited by fluoride and vanadate like in mammalian cells. B-SG was also found to be accumulated in the vacuoles. The transport rate of DNP-SG outside the cell was higher in a vacuolar-deficient strain. A significant ATP-dependent uptake of (H-3)-DNP-SG by vacuoles was found. These results indicate that S. cerevisiae transport glutathione S-conjugates both outside the cells and into the vacuoles. (C) 1996 Academic Press Limited

引用

页码：325 / 330

页数：6

共 25 条

[21] ATP-DEPENDENT EFFLUX OF 2,4-DINITROPHENYL-S-GLUTATHIONE - PROPERTIES OF 2 DISTINCT TRANSPORT-SYSTEMS IN INSIDE-OUT VESICLES FROM L1210 CELLS AND A VARIANT SUBLINE WITH ALTERED EFFLUX OF METHOTREXATE AND CHOLATE [J].