Monocyte chemoattractant protein-1 in obesity and type 2 diabetes.: Insulin sensitivity study

被引:38
作者
Chacon, Matilde R.
Fernandez-Real, Jose Manuel
Richart, Cristobal
Megia, Ana
Gomez, Jose Manuel
Miranda, Merce
Caubet, Enric
Pastor, Rosa
Masdevall, Carles
Vilarrasa, Nuria
Ricard, Wifredo
Vendrell, Joan
机构
[1] Univ Hosp Tarragona Joan XXIII, Res Unit, E-43007 Tarragona, Spain
[2] Univ Rovira & Virgili, Univ Hosp Tarragona Joan XXIII, Pere Virgili Inst, Res Dept, Tarragona, Spain
[3] Univ Hosp Josep Trueta, Endocrinol & Diab Unit, Girona, Spain
[4] Univ Hosp Bellvitge, Endocrinol & Diab Unit, Barcelona, Spain
[5] St Pauls Hosp, Surg Serv, Tarragona, Spain
[6] Univ Hosp Bellvitge, Surg Serv, Barcelona, Spain
关键词
monocyte chemoattractant protein-1; adipose tissue; insulin sensitivity; type; 2; diabetes;
D O I
10.1038/oby.2007.578
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Objective: Our goal was to test any association between human plasma circulating levels of monocyte chemoattractant protein-1 (cMCP-1) and insulin resistance and to compare monocyte chemoattractant protein-1 (MCP-1) adipose tissue gene expression and cMCP-1 in relation with inflammatory markers. Research Methods and Procedures: cMCP-1 was measured in n = 116 consecutive control male subjects to whom an insulin sensitivity (S) test was performed. Circulating levels of soluble CD14, soluble tumor necrosis factor receptor type 2 (sTNFR2), soluble interleukin-6 (sIL-6), and adiponectin also were measured. Subcutaneous adipose tissue samples were obtained from n = 107 non-diabetic and type 2 diabetic subjects with different degrees of obesity. Real-time polymerase chain reaction was used to measure gene expression of MCP-1, CD68, tumor necrosisfactor-alpha (TNF-alpha), and its receptor TNFR2. Results: In the Si study, no independent effect of cMCP-1 levels on insulin sensitivity was observed. In the expression study, in non-diabetic subjects, MCP-1 mRNA had a positive correlation with BMI (r = 0.407, p = 0.003), TNF-alpha mRNA (r = 0.419, p = 0.002), and TNFR2 mRNA (r = 0.410, p = 0.003). In these subjects, cMCP-1 was found to correlate with waist-to-hip ratio (r = 0.322, p = 0.048). In patients with type 2 diabetes, MCP-1 mRNA was up-regulated compared with non-diabetic subjects. TNF-a mRNA was found to independently contribute to MCP-1 mRNA expression. In this group, CD68 mRNA was found to correlate with BMI (r = 0.455, p = 0.001). Discussion: cMCP-1 is not associated with insulin sensitivity in apparently healthy men. TNF-alpha is the inflammatory cytokine associated with MCP-1 expression in subcutaneous adipose tissue.
引用
收藏
页码:664 / 672
页数:9
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