The structure and mechanism of the Mycobacterium tuberculosis cyclodityrosine synthetase

被引：54

作者：

Vetting, Matthew W. ^{[1
]}

Hegde, Subray S. ^{[1
]}

Blanchard, John S. ^{[1
]}

机构：

[1] Albert Einstein Coll Med, Dept Biochem, Bronx, NY 10467 USA

来源：

NATURE CHEMICAL BIOLOGY | 2010年 / 6卷 / 11期

基金：

美国国家卫生研究院;

关键词：

BIOSYNTHETIC GENE-CLUSTER; ASPERGILLUS-FUMIGATUS; IN-VITRO; PEPTIDE; CYTOCHROME-P450; GLIOTOXIN; CYP121; VIVO;

D O I：

10.1038/NCHEMBIO.440

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Mycobacterium tuberculosis enzyme Rv2275 catalyzes the formation of cyclo(L-Tyr-L-Tyr) using two molecules of Tyr-tRNA(Tyr) as substrates. The three-dimensional (3D) structure of Rv2275 was determined to 2.0-angstrom resolution, revealing that Rv2275 is structurally related to the class Ic aminoacyl-tRNA synthetase family of enzymes. Mutagenesis and radioactive labeling suggests a covalent intermediate in which L-tyrosine is transferred from Tyr-tRNA(Tyr) to an active site serine (Ser88) by transesterification with Glu233 serving as a critical base, catalyzing dipeptide bond formation.

引用

页码：797 / 799

页数：3

共 16 条

[11] Characterization of Active Site Structure in CYP121 A CYTOCHROME P450 ESSENTIAL FOR VIABILITY OF MYCOBACTERIUM TUBERCULOSIS H37Rv [J].

McLean, Kirsty J. ;

Carroll, Paul ;

Lewis, D. Geraint ;

Dunford, Adrian J. ;

Seward, Harriet E. ;

Neeli, Rajasekhar ;

Cheesman, Myles R. ;

Marsollier, Laurent ;

Douglas, Philip ;

Smith, W. Ewen ;

Rosenkrands, Ida ;

Cole, Stewart T. ;

Leys, David ;

Parish, Tanya ;

Munro, Andrew W. .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2008, 283 (48) :33406-33416

[12] BICYCLOMYCIN, A NEW ANTIBIOTIC .3. IN-VITRO AND IN-VIVO ANTIMICROBIAL ACTIVITY [J].

NISHIDA, M ;

MINE, Y ;

KUWAHARA, S ;

MATSUBARA, T ;

GOTO, S .

JOURNAL OF ANTIBIOTICS, 1972, 25 (10) :582-+

[13]

PURDIE JE, 1973, J CHEM SOC P2, V2, P1845

[14] Exploring the impact of different thioesterase domains for the design of hybrid peptide synthetases [J].

Schwarzer, D ;

Mootz, HD ;

Marahiel, MA .

CHEMISTRY & BIOLOGY, 2001, 8 (10) :997-1010

[15] PEPTIDE DECOMPOSITION IN THE NEUTRAL PH-REGION VIA THE FORMATION OF DIKETOPIPERAZINES [J].

STEINBERG, SM ;

BADA, JL .

JOURNAL OF ORGANIC CHEMISTRY, 1983, 48 (13) :2295-2298

[16] Lactobacillus plantarum MiLAB 393 produces the antifungal cyclic dipeptides cyclo(L-Phe-L-Pro) and cyclo(L-Phe-trans-4-OH-L-Pro) and 3-phenyllactic acid [J].

Ström, K ;

Sjögren, J ;

Broberg, A ;

Schnürer, J .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2002, 68 (09) :4322-4327

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