The structure and mechanism of the Mycobacterium tuberculosis cyclodityrosine synthetase

被引：54
作者：
Vetting, Matthew W. [1 ]
Hegde, Subray S. [1 ]
Blanchard, John S. [1 ]
机构：
[1] Albert Einstein Coll Med, Dept Biochem, Bronx, NY 10467 USA
来源：
NATURE CHEMICAL BIOLOGY | 2010年 / 6卷 / 11期
基金：
美国国家卫生研究院;
关键词：
BIOSYNTHETIC GENE-CLUSTER; ASPERGILLUS-FUMIGATUS; IN-VITRO; PEPTIDE; CYTOCHROME-P450; GLIOTOXIN; CYP121; VIVO;
D O I：
10.1038/NCHEMBIO.440
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The Mycobacterium tuberculosis enzyme Rv2275 catalyzes the formation of cyclo(L-Tyr-L-Tyr) using two molecules of Tyr-tRNA(Tyr) as substrates. The three-dimensional (3D) structure of Rv2275 was determined to 2.0-angstrom resolution, revealing that Rv2275 is structurally related to the class Ic aminoacyl-tRNA synthetase family of enzymes. Mutagenesis and radioactive labeling suggests a covalent intermediate in which L-tyrosine is transferred from Tyr-tRNA(Tyr) to an active site serine (Ser88) by transesterification with Glu233 serving as a critical base, catalyzing dipeptide bond formation.
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收藏
页码：797 / 799
页数：3
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