Injectable In Situ Self-Cross-Linking Hydrogels Based on Poly(L-glutamic acid) and Alginate for Cartilage Tissue Engineering

被引:200
作者
Yan, Shifeng [1 ,3 ]
Wang, Taotao [1 ]
Feng, Long [1 ]
Zhu, Jie [1 ]
Zhang, Kunxi [1 ]
Chen, Xuesi [4 ]
Cui, Lei [2 ]
Yin, Jingbo [1 ]
机构
[1] Shanghai Univ, Dept Polymer Mat, Shanghai 200444, Peoples R China
[2] Capital Med Univ, Beijing Shijitan Hosp, Med Sci & Res Ctr, Beijing 100038, Peoples R China
[3] Fudan Univ, State Key Lab Mol Engn Polymers, Shanghai 200433, Peoples R China
[4] Chinese Acad Sci, Changchun Inst Appl Chem, Key Lab Polymer Ecomat, Changchun 130022, Peoples R China
基金
中国国家自然科学基金; 上海市自然科学基金;
关键词
HYALURONIC-ACID; STEREOCOMPLEX FORMATION; BIOMEDICAL APPLICATIONS; BIOMIMETIC MATERIALS; PROTEIN DELIVERY; PART; CHITOSAN; SCAFFOLDS; DEXTRAN; REGENERATION;
D O I
10.1021/bm501313t
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Injectable hydrogels as an important biomaterial class have been widely used in regenerative medicine. A series of injectable poly(l-glutamic acid)/alginate (PLGA/ALG) hydrogels were fabricated by self-cross-linking of hydrazide-modified poly(L-glutamic acid) (PLGA-ADH) and aldehyde-modified alginate (ALG-CHO). Both the degree of PLGA modification and the oxidation degree of ALG-CHO could be adjusted by the amount of activators and sodium periodate, respectively. The effect of the solid content of the hydrogels and oxidation degree of ALG-CHO on the gelation time, equilibrium swelling, mechanical properties, microscopic morphology, and in vitro degradation of the hydrogels was examined. Encapsulation of rabbit chondrocytes within hydrogels showed viability of the entrapped cells and good biocompatibility of the injectable hydrogels. A preliminary study exhibited injectability and rapid in vivo gel formation, as well as mechanical stability, cell ingrowth, and ectopic cartilage formation. The injectable PLGA/ALG hydrogels demonstrated attractive properties for future application in a variety of pharmaceutical delivery and tissue engineering, especially in cartilage tissue engineering.
引用
收藏
页码:4495 / 4508
页数:14
相关论文
共 55 条
[1]   Investigation of gelation mechanism of an injectable hydrogel based on chitosan by rheological measurements for a drug delivery application [J].
Aliaghaie, Marzieh ;
Mirzadeh, Hamid ;
Dashtimoghadam, Erfan ;
Taranejoo, Shahrouz .
SOFT MATTER, 2012, 8 (27) :7128-7137
[2]   The effect of elastin on chondrocyte adhesion and proliferation on poly (ε-caprolactone)/elastin composites [J].
Annabi, Nasim ;
Fathi, Ali ;
Mithieux, Suzanne M. ;
Martens, Penny ;
Weiss, Anthony S. ;
Dehghani, Fariba .
BIOMATERIALS, 2011, 32 (06) :1517-1525
[3]   Self-cross-linking biopolymers as injectable in situ forming biodegradable scaffolds [J].
Balakrishnan, B ;
Jayakrishnan, A .
BIOMATERIALS, 2005, 26 (18) :3941-3951
[4]   Human immune responses to porcine xenogeneic matrices and their extracellular matrix constituents in vitro [J].
Bayrak, Alexandra ;
Tyralla, Maria ;
Ladhoff, Juliane ;
Schleicher, Martina ;
Stock, Ulrich A. ;
Volk, Hans-Dieter ;
Seifert, Martina .
BIOMATERIALS, 2010, 31 (14) :3793-3803
[5]   Tissue reactions of in situ formed dextran hydrogels crosslinked by stereocomplex formation after subcutaneous implantation in rats [J].
Bos, GW ;
Hennink, WE ;
Brouwer, LA ;
den Otter, W ;
Veldhuis, TFJ ;
van Nostrum, CF ;
van Luyn, MJA .
BIOMATERIALS, 2005, 26 (18) :3901-3909
[6]   Synthesis and characterization of dextran-peptide-methotrexate conjugates for tumor targeting via mediation by matrix metalloproteinase II and matrix metalloproteinase IX [J].
Chau, Y ;
Tan, FE ;
Langer, R .
BIOCONJUGATE CHEMISTRY, 2004, 15 (04) :931-941
[7]   Encapsulation of lycopene extract from tomato pulp waste with gelatin and poly(γ-glutamic acid) as carrier [J].
Chiu, Y. T. ;
Chiu, C. P. ;
Chien, J. T. ;
Ho, G. H. ;
Yang, J. ;
Chen, B. H. .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2007, 55 (13) :5123-5130
[8]   Polyelectrolyte complexes based on chitosan and poly(L-glutamic acid) [J].
Dai, Zhengzhan ;
Yin, Jingbo ;
Yan, Shifeng ;
Cao, Tian ;
Ma, Jia ;
Chen, Xuesi .
POLYMER INTERNATIONAL, 2007, 56 (09) :1122-1127
[9]   Natural polymers for gene delivery and tissue engineering [J].
Dang, Jiyoung M. ;
Leong, Kam W. .
ADVANCED DRUG DELIVERY REVIEWS, 2006, 58 (04) :487-499
[10]   Biodegradable hydrogels based on stereocomplex formation between lactic acid oligomers grafted to dextran [J].
de Jong, SJ ;
De Smedt, SC ;
Demeester, J ;
van Nostrum, CF ;
Kettenes-van den Bosch, JJ ;
Hennink, WE .
JOURNAL OF CONTROLLED RELEASE, 2001, 72 (1-3) :47-56